Direct-Blot™ HRP anti-V5-tag Antibody

Pricing & Availability
Clone
7/4 (See other available formats)
Other Names
GKPIPNPLLGLDST epitope tag
Isotype
Mouse IgG2b, κ
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Product Citations
publications
7-4_DB-HRP_V5-tag_Antibody_031319
0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) and total cell lysates (15 µg total protein) from untransformed BL21 E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:4000 dilution of Direct-Blot™ HRP anti-V5-tag antibody, clone 7/4, overnight at 4°C. Proteins were visualized by chemiluminescence detection. Lane M: Molecular Weight marker.
  • 7-4_DB-HRP_V5-tag_Antibody_031319
    0.5 µL of Recombinant Posi-Tag Epitope Tag Protein (Cat No. 931301) and total cell lysates (15 µg total protein) from untransformed BL21 E. coli cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 1:4000 dilution of Direct-Blot™ HRP anti-V5-tag antibody, clone 7/4, overnight at 4°C. Proteins were visualized by chemiluminescence detection. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
680604 25 µL $85
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680603 100 µL $290
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Description

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The V5 tag is a 14 amino acid peptide derived from a small epitope (Pk) on the P and V proteins of simian virus 5 (SV5), a member of the paramyxovirus family. Because of its small size, the addition of a V5 tag sequence (GKPIPNPLLGLDST) to a given gene creates a stable fusion product that does not appear to interfere with the biochemical properties of the protein. Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.

Product Details
Technical data sheet

Product Details

Reactivity
GKPIPNPLLGLDST tag epitope
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

RRID
AB_2734532 (BioLegend Cat. No. 680604)
AB_2572008 (BioLegend Cat. No. 680603)

Antigen Details

Structure
14 amino acid peptide (GKPIPNPLLGLDST)
Function
The V5 tag can be used to detect the expression of recombinant proteins in bacteria, yeast, insects, and mammalian cell systems.
Biology Area
Cell Biology
Antigen References

1. Uhlen M, et al. 1990. Meth Enzymol. 185:129.
2. Hanke T, et al. 1995. J. Virol. Methods 53:149.
3. Southern JA, et al. 1991. J. Gen. Virol. 72:1551.
4. Thomas SM, et al. 1988. Cell 54:891.

Gene ID
NA
UniProt
View information about V5-tag on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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