Purified anti-V5 Epitope Tag Antibody (Previously Covance catalog# PRB-189P)

Pricing & Availability
Clone
Poly29038 (See other available formats)
Other Names
GKPIPNPLLGLDST epitope tag, GKPIPNPLLGLDST tag, Protein Rev, Regulator of expression of viral proteins, Rev, V5 epitope tag
Previously
Covance Catalog# PRB-189P
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly29038_PURE_V5_Epitope_Tag_Antibody_WB_112817
Western blot analysis of cell lysates from HeLa cells transfected without or with V5-tagged plasmid using V5 Rabbit primary antibody (clone Poly 29038, 1:10000 dilution, 0.1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807, 1:8000 dilution). was used as a loading control.
  • Poly29038_PURE_V5_Epitope_Tag_Antibody_WB_112817
    Western blot analysis of cell lysates from HeLa cells transfected without or with V5-tagged plasmid using V5 Rabbit primary antibody (clone Poly 29038, 1:10000 dilution, 0.1 µg/ml) and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807, 1:8000 dilution). was used as a loading control.
Cat # Size Price Quantity Avail. Save
903802 25 µl $75
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903801 100 µl $281
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Description

The V5 epitope tag is used to label a protein of interest so that the tagged protein can be analyzed and visualized. Antibodies against V5 epitope tag recognize tagged proteins and allow for specific detection in several immunochemical assays such as western blotting, ELISAs and immunostaining techniques.

Product Details
Technical data sheet

Product Details

Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Polyclonal V5 antisera was raised against a synthetic peptide representing amino acid residues 95 to 108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian parainfluenza virus 5 conjugated to KLH.
Formulation
Phosphate-buffered solution with 0.1% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
ELISA, IHC - Validated
Purification - Reported in literature

Recommended Usage

The optimal working dilution should be determined for each specific assay condition.
WB: 0.1 - 0.2 ug/ml (1:5000 - 1:10000)
IHC: 1:100 - 400

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry and ELISA.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

The antisera was purified via affinity chromatography using the peptide immobilized on solid support.


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. J Dixelius, et al. 2003. J. Biol. Chem. 278:40973.
RRID
AB_2734673 (BioLegend Cat. No. 903802)
AB_2565034 (BioLegend Cat. No. 903801)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about V5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 01/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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