Purified anti-c-Myc Antibody

Pricing & Availability
Clone
9E10 (See other available formats)
Other Names
Oncogene Myc, Myc proto-oncogene protein, proto-oncogene c-Myc
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
9E10_PURE_c-Myc_Antibody_WB_062618
Total cell lysates (15 µg protein) from 293E cells transfected with Multi-tag (lane 1) or non-transfected cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-c-Myc antibody (1:500 dilution), clone 9E10 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-Myc antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower blot). Lane M: Molecular weight ladder.
  • 9E10_PURE_c-Myc_Antibody_WB_062618
    Total cell lysates (15 µg protein) from 293E cells transfected with Multi-tag (lane 1) or non-transfected cells (lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1 µg/mL purified anti-c-Myc antibody (1:500 dilution), clone 9E10 (upper blot). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-c-Myc antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower blot). Lane M: Molecular weight ladder.
Cat # Size Price Quantity Avail. Save
626801 25 µg $85
Check Availability


Need larger quantities of this item?
Request Bulk Quote
626802 100 µg $185
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical data sheet

Product Details

Reactivity
Endogenous Human c-Myc; fusion protein with c-Myc tag (EQKLISEEDL, around 1.2kD) in all species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP, IHC-P, Purification - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Kondo S, et al. 2011. J Virol. 85:11255. PubMed
  2. Thibodeau M 2012. J Immunol. 188:4959. PubMed
  3. Decarpentrie F, et al. 2012. Hum Mol Genet. 2.702083333. PubMed
  4. Peters B, et al. 2012. Microbiology. 158:2975. PubMed
  5. Robu M, et al. 2013. Proc Natl Acad Sci U S A. 110:1658. PubMed
  6. Eilert E, et al. 2013. J Biotechnol. 167:85. PubMed
  7. Bi J, et al. 2013. Am J Physiol Renal Physiol. 305:532. PubMed
  8. Poe J, et al. 2013. J Exp Med. 511:57. PubMed
  9. Shkoporov A, et al. 2015. FEMS Microbiol Lett. 362:83. PubMed
  10. Rai R, et al. 2015. Genetics. 201: 989 - 1016. PubMed
  11. Muthusamy B, et al. 2015. PLoS Biol. 13(12): e1002325. PubMed
  12. Kennedy J, et al. 2015. PLoS One. 10: 0145466. PubMed
  13. Wang R, et al. 2016. Nat Commun. 7:10269. PubMed
  14. Alonso-Rodríguez E, et al. 2016. J Biol Chem. 291: 5461 - 5472. PubMed
  15. García R, et al. 2016. J Cell Sci. 129: 1649-1660. PubMed
  16. Dowd G, et al. 2016. Infect Immun . 84: 1826 - 1841. PubMed
  17. Syed S, et al. 2016. PLoS Genet. 12:e1006451. PubMed
  18. GÉnier S, et al. 2016. Mol Biol Cell. 27(24):3800-3812. PubMed
  19. Bloch N, et al. 2017. J Biol Chem. 292(3):1068-1080. PubMed
  20. Huang X, et al. 2017. PLoS Pathogens. 13(6):e1006414. PubMed
  21. Lin JR 2018. eLife. 7: e31657. PubMed
  22. Omer S 2018. eLife. 7: e36745. PubMed
  23. Lao JP, et al. 2018. G3 (Bethesda). 8:3931. PubMed
  24. Walch L, et al. 2018. Sci Rep. 8:17121. PubMed
  25. Alameda JP, et al. 2019. Aging (Albany NY). 11:127. PubMed
  26. Cox NJ, et al. 2018. Biochemistry. 57:91. PubMed
  27. Case BA, et al. 2018. Bioconjug Chem. 29:1646. PubMed
  28. Farmer GE, et al. 2019. Sci Rep. 9:8820. PubMed
Publication Library
RRID
AB_2235686 (BioLegend Cat. No. 626801)
AB_2148451 (BioLegend Cat. No. 626802)

Antigen Details

Structure
Highly conserved protein structure from drosophila to vertebrate. Contains HLH domain, coiled coil region, and leucine zipper domain. Human c-myc has two isoforms with predicted MW of 49 kD and 51kD.
Distribution

Nuclear protein

Function
Transcription factor, binds to DNA and activates transcription as part of a heterodimeric complex MAX. Structural rearrangements found in a number of cancers
Interaction
MAX, SMAD2, SMAD3, Pam, cdc6, BRCA1, Mlh1, p34cdc2, MAD, Sp1, and many other proteins
Modification
Glycosylation, Phosphorylation (Thr58, Ser62, Ser71)
Cell Type
Embryonic Stem Cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Stem Cells, Transcription Factors
Antigen References

1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
3. Battey J, et al. 1983. Cell 34:779.
4. Beimling P, et al. 1985. Biochemistry 24:6349.

Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Reviews

Review This Product

Related FAQs

There are no FAQs for this product.
Go To Top Version: 7    Revision Date: 08/02/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account