Direct-Blot™ HRP anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
16B12_DB-HRP_HA.11_Antibody_WB_042017
Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
  • 16B12_DB-HRP_HA.11_Antibody_WB_042017
    Total cell lysate from CHO (lane 1) and CHO stably transfected with HA tag fused protein (lane 2) (15 µg/lane) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:10,000 purified anti-HA.11 or 1:10,000 Direct-Blot™ HRP anti-HA.11 Antibody. For the purified anti-HA.11 antibody, proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading controls (lower). Lane M is the MW ladder.
Cat # Size Price Quantity Avail. Save
901520 25 µl $85
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901519 100 µl $290
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical data sheet

Product Details

Reactivity
YPYDVPDYA Tag
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:10000. The optimal dilution should be determined by titration for each individual assay of interest.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

This antibody is effective in immunoblotting (WB), immunofluorescence (IF), and immunoprecipitation (IP) of tagged proteins.

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. Pecot MY, Malhotra V. 2004. Cell. 116:99.
  2. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  3. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  4. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  5. Kolodziej P, Young R. 1991. Meth Enzymol. 194:508. (General)
  6. Field J, et al. 1988. Mol Cell Biol. 8:2159. (General)
  7. Majeed SR, et al. 2014. Nat Commun. 23:5891. (IF) PubMed
  8. Fuentes EJ, et al. 2003. J Biol Chem. 278:2118. PubMed
  9. Hatanaka H, et al. 2009. J. Biol. Chem. 23:15448. (WB) PubMed
  10. Zhang Y, et al. 2007. J. Biol. Chem. 282:13022. (WB) PubMed
  11. Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
RRID
AB_2749912 (BioLegend Cat. No. 901520)
AB_2686981 (BioLegend Cat. No. 901519)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 04/21/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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