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Spark Blue™ 550 anti-human CD4 Antibody

Pricing & Availability
Clone
SK3 (See other available formats)
Regulatory Status
RUO
Other Names
T4, Leu3a
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK3_SPKBL_550_CD4_Antibody_100319
Human peripheral blood lymphocytes were stained with CD4 (SK3) Spark Blue 550 (filled histogram) versus unstained cells (open histogram).
  • SK3_SPKBL_550_CD4_Antibody_100319
    Human peripheral blood lymphocytes were stained with CD4 (SK3) Spark Blue 550 (filled histogram) versus unstained cells (open histogram).
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344655 25 tests £98
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344656 100 tests £234
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 550 has a maximum excitation of 516 nm and a maximum emission of 540 nm.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Evans RL, et al. 1981. Immunol. 78:544
  2. Arno A et al. 1999. J. Infect. Dis. 180:56
  3. Muech M, et al. 1997. Blood 89:1364
  4. Wang L, et al. 2012. Cytometry A. 81:567. PubMed
Product Citations
  1. Kreutmair S, et al. 2021. Immunity. . PubMed
RRID
AB_2819978 (BioLegend Cat. No. 344655)
AB_2819979 (BioLegend Cat. No. 344656)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D et al. 1996. Immunol. Today 17:476.
2. Gaubin M et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD4 Reagents Request Custom Conjugation
Description Clone Applications
PerCP anti-human CD4 SK3 FC
Purified anti-human CD4 SK3 FC, IP
FITC anti-human CD4 SK3 FC
PE anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Biotin anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
APC/Cyanine7 anti-human CD4 SK3 FC
Alexa Fluor® 488 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Alexa Fluor® 700 anti-human CD4 SK3 FC
Purified anti-human CD4 (Maxpar® Ready) SK3 FC, CyTOF®
PE anti-human CD4 SK3 FC
Brilliant Violet 421™ anti-human CD4 SK3 FC
Brilliant Violet 510™ anti-human CD4 SK3 FC
Alexa Fluor® 647 anti-human CD4 SK3 FC
FITC anti-human CD4 SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
PE/Dazzle™ 594 anti-human CD4 SK3 FC
Pacific Blue™ anti-human CD4 SK3 FC
Brilliant Violet 785™ anti-human CD4 SK3 FC
Brilliant Violet 750™ anti-human CD4 SK3 FC
Brilliant Violet 605™ anti-human CD4 SK3 FC
Brilliant Violet 711™ anti-human CD4 SK3 FC
PE/Cyanine7 anti-human CD4 SK3 FC
TotalSeq™-A0045 anti-human CD4 SK3 PG
TotalSeq™-C0045 anti-human CD4 SK3 PG
PE/Cyanine5 anti-human CD4 SK3 FC
PerCP/Cyanine5.5 anti-human CD4 SK3 FC
Spark Blue™ 550 anti-human CD4 SK3 FC
Spark NIR™ 685 anti-human CD4 SK3 FC
APC anti-human CD4 SK3 FC
KIRAVIA Blue 520™ anti-human CD4 SK3 FC
APC/Fire™ 810 anti-human CD4 SK3 FC
PE/Fire™ 640 anti-human CD4 SK3 FC
PE/Fire™ 700 anti-human CD4 SK3 FC
PerCP anti-human CD4 SK3 FC
Spark Violet™ 538 anti-human CD4 Antibody SK3 FC
Spark YG™ 581 anti-human CD4 SK3 FC
Alexa Fluor® 660 anti-human CD4 SK3 FC
Spark YG™ 593 anti-human CD4 SK3 FC
PE/Fire™ 810 anti-human CD4 Antibody SK3 FC
APC/Fire™ 750 anti-human CD4 SK3 FC
Spark Blue™ 574 anti-human CD4 SK3 FC
GMP APC anti-human CD4 SK3 FC
TotalSeq™-B0045 anti-human CD4 Antibody SK3 PG
Spark Violet™ 423 anti-human CD4 Antibody SK3 FC
GMP Pacific Blue™ anti-human CD4 SK3 FC
Spark UV™ 387 anti-human CD4 SK3 FC
GMP PE anti-human CD4 SK3 FC
GMP FITC anti-human CD4 SK3 FC
Spark Red™ 718 anti-human CD4 SK3 FC
GMP PerCP anti-human CD4 SK3 FC
GMP PE/Cyanine7 anti-human CD4 SK3 FC
Go To Top Version: 2    Revision Date: 09/14/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PerCP anti-human CD4

  • Purified anti-human CD4

  • FITC anti-human CD4

  • PE anti-human CD4

  • PerCP/Cyanine5.5 anti-human CD4

  • Biotin anti-human CD4

  • PE/Cyanine7 anti-human CD4

  • APC anti-human CD4

  • APC/Cyanine7 anti-human CD4

  • Alexa Fluor® 488 anti-human CD4

  • Pacific Blue™ anti-human CD4

  • Alexa Fluor® 700 anti-human CD4

  • Purified anti-human CD4 (Maxpar® Ready)

  • PE anti-human CD4

  • Brilliant Violet 421™ anti-human CD4

  • Brilliant Violet 510™ anti-human CD4

  • Alexa Fluor® 647 anti-human CD4

  • FITC anti-human CD4

  • APC/Fire™ 750 anti-human CD4

  • PE/Dazzle™ 594 anti-human CD4

  • Pacific Blue™ anti-human CD4

  • Brilliant Violet 785™ anti-human CD4

  • Brilliant Violet 750™ anti-human CD4

  • Brilliant Violet 605™ anti-human CD4

  • Brilliant Violet 711™ anti-human CD4

  • PE/Cyanine7 anti-human CD4

  • TotalSeq™-A0045 anti-human CD4

  • TotalSeq™-C0045 anti-human CD4

  • PE/Cyanine5 anti-human CD4

  • PerCP/Cyanine5.5 anti-human CD4

  • Spark Blue™ 550 anti-human CD4

  • Spark NIR™ 685 anti-human CD4

  • APC anti-human CD4

  • KIRAVIA Blue 520™ anti-human CD4

  • APC/Fire™ 810 anti-human CD4

  • PE/Fire™ 640 anti-human CD4

  • PE/Fire™ 700 anti-human CD4

  • PerCP anti-human CD4

  • Spark Violet™ 538 anti-human CD4 Antibody

  • Spark YG™ 581 anti-human CD4

  • Alexa Fluor® 660 anti-human CD4

  • Spark YG™ 593 anti-human CD4

  • PE/Fire™ 810 anti-human CD4 Antibody

  • APC/Fire™ 750 anti-human CD4

  • Spark Blue™ 574 anti-human CD4

  • GMP APC anti-human CD4

  • TotalSeq™-B0045 anti-human CD4 Antibody

  • Spark Violet™ 423 anti-human CD4 Antibody

  • GMP Pacific Blue™ anti-human CD4

  • Spark UV™ 387 anti-human CD4

  • GMP PE anti-human CD4

  • GMP FITC anti-human CD4

  • Spark Red™ 718 anti-human CD4

  • GMP PerCP anti-human CD4

  • GMP PE/Cyanine7 anti-human CD4
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