Recombinant Mouse IL-6 (carrier-free)

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Other Names
Interleukin-6, Interferon-β2, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
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rm_IL-6_112108
Mouse IL-6 induces the proliferation of mouse 7TD1 cells.
  • rm_IL-6_112108
    Mouse IL-6 induces the proliferation of mouse 7TD1 cells.
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Cat # Size Price Quantity Avail. Save
575702 10 µg £86
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575704 25 µg £154
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575706 100 µg £376
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575708 500 µg £1,094
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Description

IL-6 is a multifunctional cytokine that can regulate various immune and inflammatory responses. Several studies have suggested a crucial role for IL-6 in angiogenesis. The use of mice deficient in IL-6 (-/-) demonstrated a critical role for this protein in a mouse model of lung angiogenesis. IL-6 has been shown to cause proliferation and migration of systemic endothelial cells in culture (1). The classical responsiveness to IL-6 is governed by a receptor complex consisting of two membrane-bound subunits, an 80-kD cognate chain (IL-6R), and a ubiquitously expressed 130-kD β-chain receptor (gp130) which acts as the universal signal-transducing element for all IL-6 family cytokines (2). Alternatively, IL-6 regulation of leukocyte trafficking relies upon signaling via its soluble IL-6R (termed IL-6 trans-signaling) (3). IL-6 plays a major role in regulating neutrophil clearance during acute peritoneal inflammation; as a result of specific down-regulation of neutrophil-attracting chemokine (CXCL1/KC) production (4). IL-6 is a key factor that reciprocally regulates Th17 and Foxp3(+) Treg differentiation by inhibition of TGF-beta induced Foxp3 and induction of RORgammat, a Th17 lineage-specific transcription factor (5).

Product Details
Technical Data Sheet (pdf)

Product Details

Source
Mouse IL-6, amino acids Phe25-Thr211 (Accession# NM_031168), was expressed in E. coli.
Molecular Mass
The 188 amino acid N-terminal methionylated recombinant protein has a predicted molecular mass of 21,866 Da. The DTT-reduced protein migrates at approximately 22 kD and the non-reduced protein migrates at approximately 21.5 kD by SDS-PAGE.
Purity
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution in Sodium Acetate and EDTA.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
Concentration
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial (please contact technical support for concentration, or use our Lookup tool if you have a lot number.)
Please note, new lots of the 100 µg size will be lot-specific and may differ from previous lots that had a fixed concentration.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
The ED50 is < 0.01 ng/ml, corresponding to a specific activity of > 1 x 108 units/mg, as determined by the dose dependent stimulation of 7TD1 cell proliferation.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Additional Product Notes

Get a 50% discount on this product when purchased in our Activation Bundles. Restrictions apply. Learn more…

Application References

(PubMed link indicates BioLegend citation)
  1. Wang Q, et al. 2010. J. Immunol. 185:834. PubMed
  2. Suzuki T, et al. 2011. J. Biol Chem. 286:31263. PubMed
  3. Alcaide P, et al. 2012. J. Immunol. 188:1421. PubMed
  4. Hilberath JN, et al. 2011. FASEB J. 25:1827. PubMed
  5. Allen MJ, et al. 2014. J Nutr. 144:1306. PubMed
Product Citations
  1. Khosravi F, Loeian S, Panchapakesan B 2017. Biosensors. 10.3390/bios7020017. PubMed
  2. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  3. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  4. Ren W, et al. 2018. Mucosal Immunol. 12:531. PubMed
  5. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  6. Gumusoglu SB, et al. 2017. Brain Behav Immun. 65:274. PubMed
  7. Seboko AM, et al. 2019. Front Physiol. 9:1812. PubMed
  8. Xu Y, et al. 2019. JCI Insight. 4:5. PubMed
  9. Li W, et al. 2019. Front Immunol. 10:833. PubMed
  10. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  11. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  12. Ramstead AG, et al. 2020. Cell Rep. 30:2889. PubMed
  13. Tonsawan P, et al. 2019. Am J Physiol Cell Physiol. 317:C1048. PubMed
  14. Wang Q, et al. 2010. J Immunol. 185:834. PubMed
  15. Suzuki T, et al. 2011. J Biol Chem. 286:31263. PubMed
  16. Allen M, et al. 2014. J Nutr. 144:1306. PubMed
  17. Bremser A, et al. 2015. PLoS One. 10: 0137393. PubMed
  18. Lin C, et al. 2016. J Exp Med. 213: 251 - 271. PubMed
  19. Singh R, et al. 2017. J Immunol. 10.4049/jimmunol.1602010. PubMed

Antigen Details

Distribution

IL-6 is released by activated T cells, B cells, monocytes, macrophages, fibroblasts, epithelial cells, and endothelial cells.

Function
Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Interaction
T cells, B cells, hepatocytes, cholinergic neurons
Bioactivity
Hematopoiesis, antigen-specific immune responses, inflammatory reactions, acute phase reactions
Receptors
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130) and soluble IL-6R
Cell Type
Hematopoietic stem and progenitors
Biology Area
Stem Cells, Immunology, Innate Immunity, Cell Biology
Molecular Family
Cytokines/Chemokines
Antigen References

1. McClintock JE and Wagner EM. 2005. 99:861-866
2. Murakami M. 1993. Science 260:1808-1810.
3. Jones SA,et al. 2001.J. FASEB. 15:43-58.
4. Fielding CA,et al. 2008. J. Immunol. 181:2189-2195.
5. Sonderegger I,et al. 2008. Eur. J. Immunol. 38:1833-1838.

Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
Use formula Specific activity (Units/mg) = 10e6/ ED50 (ng/mL)
How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Go To Top Version: 3    Revision Date: 11/21/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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