Purified anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
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Product Citations
publications
RM4-5_Pure_030206
C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram).
  • RM4-5_Pure_030206
    C57BL/6 mouse splenocytes stained with purified CD4 (clone RM4-5) (filled histogram) or purified rat IgG2a, κ isotype control (open histogram).
  • RM4-5_Pure_CD4_Antibody_2_100219
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of purified anti-mouse CD4 (clone RM4-5), and anti-mouse CD3ε (clone 500A2) Alexa Fluor® 594 (green) overnight at 4°C, followed by 2.5 µg/mL of Alexa Fluor® 647 anti-rat IgG (clone Poly4054) (red) for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
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100505 50 µg £20
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100506 500 µg £53
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF®, IHC-F - Verified
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  2. Schuhmann MK, et al. 2020. Circ Res. 127:1023. PubMed
  3. Costa B, et al. 2021. Cancers (Basel). 13:00. PubMed
  4. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  5. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  6. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  7. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
  8. Oyarce K, et al. 2018. Front Immunol. 9:112. PubMed
  9. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  10. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  11. Deng W et al. 2019. Cell reports. 27(6):1755-1768 . PubMed
  12. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  13. Van De Velde LA, et al. 2017. J Biol Chem. 292:15:00. PubMed
  14. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  15. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  16. Bignon A, et al. 2017. Front Immunol. 0.722222222. PubMed
  17. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  18. Goel S, et al. 2017. Nature. 548:471. PubMed
  19. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  20. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  21. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  22. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  23. Doorduijn EM, et al. 2018. Front Immunol. 0.416666667. PubMed
  24. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  25. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  26. Lee YJ, et al. 2018. FASEB J. 32:4658. PubMed
  27. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
  28. Tan CL, et al. 2018. Immunohorizons. 0.248611111. PubMed
  29. Atif SM, et al. 2019. JCI Insight. 4:e125494. PubMed
  30. Stebegg M, et al. 2019. Nat Commun. 2.113194444. PubMed
  31. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  32. Do DC, et al. 2019. JCI Insight. 4:e126832. PubMed
  33. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
  34. Fritz Y, et al. 2017. J Invest Dermatol. 137:696. PubMed
  35. Williams SK, et al. 2018. Sci Rep. 8:13628. PubMed
  36. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  37. Abdel-Gadir A, et al. 2019. Nat Med. 25:1164. PubMed
  38. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  39. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  40. Heinrich A, et al. 2020. Cell Rep. 31:107513. PubMed
  41. Chihara N, et al. 2018. Nature. 558:454. PubMed
  42. King IL, et al. 2017. Mucosal Immunol. 10:1160. PubMed
  43. Yoon BH, et al. 2018. Mol Cells. 41:953. PubMed
  44. Umemoto T, et al. 2017. EMBO J. 36:2390. PubMed
  45. Yi J, et al. 2019. Mol Cells. 42:228. PubMed
  46. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  47. Shin J, et al. 2018. Diabetes. 67:1068. PubMed
  48. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  49. Vitiello GA, et al. 2018. Clin Cancer Res. 24:972. PubMed
  50. Shi B, et al. 2018. J Immunol. 200:586. PubMed
  51. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  52. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
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RRID
AB_312708 (BioLegend Cat. No. 100505)
AB_312709 (BioLegend Cat. No. 100506)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 10/02/2019

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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