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Alexa Fluor® 700 anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Regulatory Status
RUO
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
RM4-5_Alx700_020608
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
  • RM4-5_Alx700_020608
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
Compare all formats See Alexa Fluor® 700 spectral data
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100536 100 µg £148
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 100575 and 100576).

Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Wu SY, et al. 2022. Mol Cancer. 21:84. PubMed
  3. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  4. Xu ZH, et al. 2021. Mediators Inflamm. 2021:8856326. PubMed
  5. Singh S, et al. 2019. PLoS Pathog. 15:e1007460. PubMed
  6. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  7. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  8. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  9. Best SA, et al. 2018. Cell Metab. 27:935. PubMed
  10. Qiu F, et al. 2022. J Cancer. 13:2893. PubMed
  11. Sandborn WJ, et al. 2021. Gastroenterology. 161:1853. PubMed
  12. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  13. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
  14. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  15. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  16. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  17. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  18. Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed
  19. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  20. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  21. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  22. Hanna CC, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  23. Jain RW, et al. 2018. Cell Rep. 25:3342. PubMed
  24. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  25. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  26. Cosentino K, et al. 2022. Mol Cell. 82:933. PubMed
  27. Altin J, et al. 2011. J Allergy Clin Immunol. 127:1277. PubMed
  28. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  29. Siegemund S, et al. 2015. PLoS One. 10:124661. PubMed
  30. Sadik A, et al. 2020. Cell. 1252:182. PubMed
  31. Nelson CE et al. 2019. Cell Rep. 28(12):3092-3104 . PubMed
  32. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  33. Fernández-Orth J, et al. 2020. Eur J Immunol. . PubMed
  34. Amezcua Vesely MC, et al. 2020. Cell. 178(5):1176-1188.e15.. PubMed
  35. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  36. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  37. Schulze B, et al. 2016. Pathog Dis. 74: ftw020. PubMed
  38. Hendrikx S et al. 2019. Cell reports. 26(5):1227-1241 . PubMed
  39. Bonavita E, et al. 2020. Immunity. 1215:53. PubMed
  40. Mykkänen O, et al. 2014. PLoS One. 9:114790. PubMed
  41. Regli IB, et al. 2020. Cell Reports. 31(10):107746. PubMed
  42. Fleskens V, et al. 2019. Cell Rep. 26:3600. PubMed
  43. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  44. Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
  45. Ito Y, et al. 2021. Cell Reports. 35(4):109052. PubMed
  46. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_493701 (BioLegend Cat. No. 100536)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Dendritic cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 5    Revision Date: 10/10/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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