- 2B8 (See other available formats)
- Regulatory Status
- Other Names
- c-KIT, Stem Cell Factor Receptor (SCFR)
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse bone marrow mast cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
- Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
- Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2563710 (BioLegend Cat. No. 105829)
- Ig superfamily, 145 kD
Hematopoietic stem cells, AML, mast cells
- Growth factor receptor, tyrosine kinase
- Stem Cell Factor (SCF)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.
- Gene ID
- 16590 View all products for this Gene ID
- View information about CD117 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD117 (c-Kit)
Biotin anti-mouse CD117 (c-Kit)
FITC anti-mouse CD117 (c-Kit)
PE anti-mouse CD117 (c-Kit)
PE/Cyanine5 anti-mouse CD117 (c-Kit)
Purified anti-mouse CD117 (c-Kit)
PE/Cyanine7 anti-mouse CD117 (c-Kit)
Alexa Fluor® 488 anti-mouse CD117 (c-Kit)
Alexa Fluor® 647 anti-mouse CD117 (c-Kit)
Pacific Blue™ anti-mouse CD117 (c-Kit)
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit)
PerCP anti-mouse CD117 (c-kit)
APC/Cyanine7 anti-mouse CD117 (c-kit)
Brilliant Violet 421™ anti-mouse CD117 (c-Kit)
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit)
Brilliant Violet 711™ anti-mouse CD117 (c-Kit)
Alexa Fluor® 594 anti-mouse CD117 (c-Kit)
APC/Fire™ 750 anti-mouse CD117 (c-Kit)
Brilliant Violet 510™ anti-mouse CD117 (c-Kit)
Brilliant Violet 785™ anti-mouse CD117 (c-Kit)
TotalSeq™-A0012 anti-mouse CD117 (c-kit)
Brilliant Violet 605™ anti-mouse CD117 (c-Kit)
Alexa Fluor® 700 anti-mouse CD117 (c-Kit)
TotalSeq™-B0012 anti-mouse CD117 (c-Kit)
TotalSeq™-C0012 anti-mouse CD117 (c-Kit)
Spark NIR™ 685 anti-mouse CD117 (c-kit)
Brilliant Violet 650™ anti-mouse CD117 (c-kit)