PE/Cyanine7 anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_PECy7_042408
C57BL/6 bone marrow cells stained with B220 FITC and 2B8 PE/Cyanine7
  • 2B8_PECy7_042408
    C57BL/6 bone marrow cells stained with B220 FITC and 2B8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
105813 25 µg £60
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105814 100 µg £145
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  2. Zhao D, et al. 2021. Innate Immun. 27:533. PubMed
  3. Katsumura KR, et al. 2018. Proc Natl Acad Sci U S A. 115:E10109. PubMed
  4. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  5. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  6. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  7. Shiozawa Y, et al. 2010. PLoS One. 5:e10853. PubMed
  8. Ray S, et al. 2020. J Biol Chem. 295:7113. PubMed
  9. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  10. Johnson KD, et al. 2022. Blood Adv. 6:1464. PubMed
  11. Chen X, et al. 2021. Theranostics. 11:4655. PubMed
  12. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  13. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  14. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  15. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  16. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  17. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  18. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  19. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  20. Dráberová L, et al. 2020. Front Immunol. 10:3068. PubMed
  21. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  22. Nakagawa T, et al. 2021. Cell Rep. 37:109875. PubMed
  23. Perner C, et al. 2020. Immunity. 53(5):1063-1077.e7. PubMed
  24. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  25. Nakano N, et al. 2008. J Allergy Clin Immunol. 123:74. PubMed
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  27. De S, et al. 2018. Development. 145. PubMed
  28. Mantri CK, et al. 2019. J Clin Invest. 129:1094. PubMed
  29. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  30. Chen I, et al. 2016. Sci Rep. 6:27195. PubMed
  31. Obata-Ninomiya K, et al. 2013. J Exp Med. 210:2583. PubMed
  32. Nakajima T, et al. 2021. J Exp Med. 218:. PubMed
  33. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  34. Tavakol DN, et al. 2021. Curr Protoc. 1:e275. PubMed
  35. Shen YC, et al. 2021. Nat Commun. 12:3876. PubMed
  36. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  37. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  38. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  39. Shibata F, et al. 2009. Stem Cells. 27:183. PubMed
  40. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  41. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  42. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  43. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  44. Mehta C, et al. 2021. Nucleic Acids Res. 49:9007. PubMed
  45. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
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  47. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  48. Zheng X, et al. 2015. Cell Rep. 13:1505-1518. PubMed
  49. Kanemaru K, et al. 2015. PLoS One. 10: 0144436. PubMed
  50. LeBlond ND, et al. 2020. J Lipid Res. 61:1697. PubMed
  51. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  52. Kobia FM, et al. 2020. PLoS Biol. 18:e3000850. PubMed
  53. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  54. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  55. Joseph J, et al. 2012. Mol Cancer Res. 0.681944444. PubMed
  56. Tung LT, et al. 2021. Cell Death Dis. 12:923. PubMed
  57. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  58. Gao X, et al. 2021. Nature. 589:591. PubMed
  59. Xu C, et al. 2020. Immunity. 53(2):417-428.e4. PubMed
  60. Sulsenti R, et al. 2021. Frontiers in Immunology. 12:622001. PubMed
  61. Xu H, et al. 2012. Blood. 120:3089. PubMed
  62. Mesa-Nuñez C, et al. 2022. Mol Ther Methods Clin Dev. 26:459. PubMed
  63. Yao Z, et al. 2020. Cancer Res. 80:1171. PubMed
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  65. Wilkinson AC, et al. 2019. Nature. 571:117. PubMed
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RRID
AB_313222 (BioLegend Cat. No. 105813)
AB_313223 (BioLegend Cat. No. 105814)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD117 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse CD117 (c-Kit) 2B8 FC
Biotin anti-mouse CD117 (c-Kit) 2B8 FC
FITC anti-mouse CD117 (c-Kit) 2B8 FC
PE anti-mouse CD117 (c-Kit) 2B8 FC
PE/Cyanine5 anti-mouse CD117 (c-Kit) 2B8 FC
Purified anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
PE/Cyanine7 anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 488 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Alexa Fluor® 647 anti-mouse CD117 (c-Kit) 2B8 FC,IHC-F
Pacific Blue™ anti-mouse CD117 (c-Kit) 2B8 FC
PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) 2B8 FC
PerCP anti-mouse CD117 (c-kit) 2B8 FC
APC/Cyanine7 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 421™ anti-mouse CD117 (c-Kit) 2B8 FC,SB
Purified anti-mouse CD117 (c-Kit) (Maxpar® Ready) 2B8 FC,CyTOF®
PE/Dazzle™ 594 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 711™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 594 anti-mouse CD117 (c-Kit) 2B8 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 510™ anti-mouse CD117 (c-Kit) 2B8 FC
Brilliant Violet 785™ anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-A0012 anti-mouse CD117 (c-kit) 2B8 PG
Brilliant Violet 605™ anti-mouse CD117 (c-Kit) 2B8 FC
Alexa Fluor® 700 anti-mouse CD117 (c-Kit) 2B8 FC
TotalSeq™-B0012 anti-mouse CD117 (c-Kit) 2B8 PG
TotalSeq™-C0012 anti-mouse CD117 (c-Kit) 2B8 PG
Spark NIR™ 685 anti-mouse CD117 (c-kit) 2B8 FC
Brilliant Violet 650™ anti-mouse CD117 (c-kit) 2B8 FC
Spark Red™ 718 anti-mouse CD117 (c-kit) (Flexi-Fluor™) 2B8 FC
Go To Top Version: 2    Revision Date: 06/28/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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