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PerCP/Cyanine5.5 anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
2B8_PerCPCy55_A_081612
C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and PerCP/Cyanine5.5 rat IgG2b isotype control
  • 2B8_PerCPCy55_A_081612
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and PerCP/Cyanine5.5 rat IgG2b isotype control
  • 2B8_PerCPCy55_B_020708
    C57BL/6 mouse bone marrow cells stained with CD45R (RA3-6B2) PE and 2B8 PerCP/Cyanine5.5
Compare all formats See PerCP/Cyanine5.5 spectral data
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105823 25 µg £81
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105824 100 µg £221
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Krauss J, et al. 2015. Proc Natl Acad Sci U S A. 112: 10455-10460. PubMed
  2. Chen S, et al. 2022. Cancer Discov. :. PubMed
  3. Inoue D, et al. 2021. Nat Genet. 53:707. PubMed
  4. De Koninck M, et al. 2020. Cell Reports. 32(6):108014. PubMed
  5. Winn H 2010. Toxicol Sci. 113:207. PubMed
  6. Yoshimi A, et al. 2019. Nature. 574:273. PubMed
  7. Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
  8. Miyazaki M et al. 2017. Immunity. 46(5):818-834 . PubMed
  9. Yamada D, et al. 2014. J Immunol. 192:4112. PubMed
  10. Chillo O, et al. 2016. Cell Rep. 16:2197-2207. PubMed
  11. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  12. Ledo JH, et al. 2020. PLoS One. e0237773:15. PubMed
  13. Schuler F, et al. 2017. Nat Commun. . 10.1038/s41467-017-01850-4. PubMed
  14. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  15. Ma Y, et al. 2009. Blood. 114:4402. PubMed
  16. Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed
  17. Walker JA, et al. 2020. Immunity. 51(1):104-118. PubMed
  18. Zeng F, et al. 2021. J Invest Dermatol. 596:141. PubMed
  19. Ledo JH, et al. 2020. Mol Psychiatry. . PubMed
RRID
AB_2131598 (BioLegend Cat. No. 105823)
AB_2131597 (BioLegend Cat. No. 105824)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 06/28/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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