- 51.1 (See other available formats)
- Regulatory Status
- Other Names
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to iNKT cells analogous to MHC molecule presentation of peptides to T cells.Product Details
- Verified Reactivity
- Reported Reactivity
- Antibody Type
- Host Species
- Human CD1d-Fc fusion
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
"Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. "
- Application Notes
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
- Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
- Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
AB_2562409 (BioLegend Cat. No. 350311)
AB_2562410 (BioLegend Cat. No. 350312)
- MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
- Present lipid antigens to iNKT cells
- When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
- Cell Type
- B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, MHC Antigens, TCRs
- Antigen References
1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.
- Gene ID
- 912 View all products for this Gene ID
- View information about CD1d on UniProt.org
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
|Purified anti-human CD1d||51.1||FC, IHC-F, IP, Stim, WB|
|PE anti-human CD1d||51.1||FC|
|APC anti-human CD1d||51.1||FC|
|PE/Cyanine7 anti-human CD1d||51.1||FC|
|PerCP/Cyanine5.5 anti-human CD1d||51.1||FC|
|Brilliant Violet 510™ anti-human CD1d||51.1||FC|
|Brilliant Violet 421™ anti-human CD1d||51.1||FC|
|TotalSeq™-A0164 anti-human CD1d||51.1||PG|
|TotalSeq™-C0164 anti-human CD1d||51.1||PG|
|Ultra-LEAF™ Purified anti-human CD1d||51.1||FC, IHC, IP, Stim, WB, Block|
|TotalSeq™-B0164 anti-human CD1d||51.1||PG|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD1d
PE anti-human CD1d
APC anti-human CD1d
PE/Cyanine7 anti-human CD1d
PerCP/Cyanine5.5 anti-human CD1d
Brilliant Violet 510™ anti-human CD1d
Brilliant Violet 421™ anti-human CD1d
TotalSeq™-A0164 anti-human CD1d
TotalSeq™-C0164 anti-human CD1d
Ultra-LEAF™ Purified anti-human CD1d
TotalSeq™-B0164 anti-human CD1d