Brilliant Violet 421™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
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Product Citations
publications
IHC_M1-70_BV421_071012
BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, stained with CD11b-BV421™ (red), B220-Alexa Fluor® 647 (blue), CD14-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
  • IHC_M1-70_BV421_071012
    BL/6 mouse lymph nodes, fixed O/N in PLP, blocked with 10% rat serum, stained with CD11b-BV421™ (red), B220-Alexa Fluor® 647 (blue), CD14-FITC (green) in 1% BSA and 0.1% Tween-20 in PBS. Images were acquired with an automated widefield microscope (Nikon Eclipse Ti) and a CCD camera (QImaging Retiga 2000R). Emitted light was collected through 440/40, 525/50, and 700/75 nm bandpass filters. Images provided by Ann Haberman and Christine Podolski, Yale University.
  • M1slash70_BV421_032911
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 421™ (filled histogram) or rat IgG2b, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
101235 125 µL £103
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101251 50 µg £157
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101236 500 µL £239
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For flow cytometric staining using the µL sizes, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Kakizaki M, Watanabe R 2017. Neuropathology. 10.1111/neup.12386. PubMed
  2. Skucha A, et al. 2018. Nat Commun. 9:1983. PubMed
  3. Geng X, et al. 2018. Aging (Albany NY). 10:1415. PubMed
  4. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  5. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  6. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  7. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  8. Xu D et al. 2018. Cell. 174(6):1477-1491 . PubMed
  9. Sabbagh MF et al. 2018. eLife. 7 pii: e36187. PubMed
  10. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  11. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  12. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  13. Yousef H, et al. 2018. Bio Protoc. 8:22. PubMed
  14. Bryson BD, et al. 2019. Nat Commun. 10:2329. PubMed
  15. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  16. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  17. Rashidi M, et al. 2019. J Immunol. 203:736. PubMed
  18. Di Mitri D, et al. 2019. Cell Rep. 28:2156. PubMed
  19. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  20. Liu J, et al. 2019. Immunity. 50:600. PubMed
  21. Li Q, et al. 2019. Neuron. 101:207. PubMed
  22. Cho C et al. 2017. Neuron. 95(5):1056-1073 . PubMed
  23. Kim I et al. 2015. Brain and behavior. 5(12):e00403 . PubMed
  24. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  25. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  26. Currier MA, et al. 2017. Oncotarget. 8:17412. PubMed
  27. Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
  28. Qi F, et al. 2019. Front Microbiol. 2.140277778. PubMed
  29. Liakopoulos V, et al. 2018. Sci Rep. 8:13964. PubMed
  30. Kodama L, et al. 2020. Nat Neurosci. 1.074305556. PubMed
  31. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
  32. O'Connor T, et al. 2020. Cancer Cell. 36(3):250-267. PubMed
  33. Ilinykh PA, et al. 2020. Cell Host & Microbe. 27(6):976-991. PubMed
  34. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  35. Liu X, et al. 2013. Diabetes. 62:4228. PubMed
  36. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  37. Price P, et al. 2015. J Immunol. 194:1164. PubMed
  38. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  39. Motozono C, et al. 2015. J Biol Chem. 290: 18924 - 18933. PubMed
  40. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  41. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  42. Ioannidis L, et al. 2016. J Immunol. 196: 1227 - 1238. PubMed
  43. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  44. Bennett M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1738 - 1746. PubMed
  45. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  46. Chai Y, et al. 2016. PLoS One. 11: 0162853. PubMed
  47. Moderzynski K, et al. 2016. PLoS Negl Trop Dis. . PubMed
  48. Reinert L, et al. 2016. Nat Commun. 7:13348. PubMed
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RRID
AB_10897942 (BioLegend Cat. No. 101235)
AB_2562904 (BioLegend Cat. No. 101251)
AB_11203704 (BioLegend Cat. No. 101236)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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