Human peripheral blood lymphocytes were stained with CD19 APC and CD1d (clone 51.1) Brilliant Violet 510™ (top) or mouse IgG2b, κ Brilliant Violet 510™ isotype control (bottom).
Human peripheral blood lymphocytes were stained with CD19 APC and CD1d (clone 51.1) Brilliant Violet 510™ (top) or mouse IgG2b, κ Brilliant Violet 510™ isotype control (bottom).
CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to iNKT cells analogous to MHC molecule presentation of peptides to T cells.
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 510™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes
Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.
Application References
(PubMed link indicates BioLegend citation)
Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution
Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells
Function
Present lipid antigens to iNKT cells
Ligand/Receptor
When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References
1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
