Brilliant Violet 510™ anti-human CD1d Antibody

Pricing & Availability
Clone
51.1 (See other available formats)
Other Names
R3G1
Isotype
Mouse IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
51.1_BV510_CD1d_Antibody_FC_1_022014.jpg
Human peripheral blood lymphocytes were stained with CD19 APC and CD1d (clone 51.1) Brilliant Violet 510™ (top) or mouse IgG2b, κ Brilliant Violet 510™ isotype control (bottom).
  • 51.1_BV510_CD1d_Antibody_FC_1_022014.jpg
    Human peripheral blood lymphocytes were stained with CD19 APC and CD1d (clone 51.1) Brilliant Violet 510™ (top) or mouse IgG2b, κ Brilliant Violet 510™ isotype control (bottom).
  • 51.1_BV510_CD1d_Antibody_FC_2_022014.jpg
See Brilliant Violet 510™ spectral data
Cat # Size Price Quantity Avail. Save
350314 100 tests £249
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD1d is a MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily. On the cell surface, CD1d forms a heterodimer with β2-microglobulin. CD1d is expressed by antigen-presenting cells such as B cells, monocytes/macrophages, dendritic cells, and some non-lymphoid cells. Cortical thymocytes express CD1d but the expression is lost in mature T cells. CD1d presents lipid antigens to iNKT cells analogous to MHC molecule presentation of peptides to T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD1d-Fc fusion
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 510™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections1, Western blotting1,2, and induction of IL-12 production by crosslinking of CD1d3.

Application References

(PubMed link indicates BioLegend citation)
  1. Exley M, et al. 2000. Immunology 100:37. (IHC, WB)
  2. Durante-Mangoni E, et al. 2004. J. Immunol. 173:2159. (WB)
  3. Yue SC, et al. 2005. P. Natl. Acad. Sci. USA 102:11811. (Stim)
RRID
AB_2563190 (BioLegend Cat. No. 350314)

Antigen Details

Structure
MHC-like, type I transmembrane protein, member of the CD1 family and the immunoglobulin superfamily
Distribution

Expressed by cortical thymocytes, monocytes, macrophages, dendritic cells, B cells (highly expressed on marginal zone B cells in particular), and in some non-lymphoid cells

Function
Present lipid antigens to iNKT cells
Ligand/Receptor
When loaded with a lipid, is recognized by the Vα14i TCR from iNKT cells
Cell Type
B cells, Dendritic cells, Macrophages, Monocytes, Thymocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, MHC Antigens, TCRs
Antigen References

1. Koch M, et al. 2005. Nat. Immunol. 6:819.
2. Liu X, et al. 2010. P. Natl. Acad. Sci. USA 107:13010.
3. Zeissig S, et al. 2010. J. Clin. Invest. 120:2889.
4. Teige A, et al. 2010. J. Immunol. 185:345.

Gene ID
912 View all products for this Gene ID
UniProt
View information about CD1d on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/21/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account