- CD28.2 (See other available formats)
- Other Names
- T44, Tp44
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.Product Details
- Human, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemical staining of acetone-fixed frozen tissue sections4, and in vitro T cell costimulation5-8. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The CD28.2 antibody co-stimulates T cell proliferation and cytokine production in the presence of suboptimal amounts of anti-CD3 antibody. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays. For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302934) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Additional Product Notes
- BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Nunes J, et al. 1993. Biochem. J. 293:835.
- Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
- Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
- Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
- Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
- Rivollier A, et al. 2004. Blood 104:4029. (Costim)
- Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
- Sheng W, et al. 2007.Elsevier 580:6819. PubMed
- Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
- Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
- Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
- Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC)
- Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
- Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
- Product Citations
AB_10644004 (BioLegend Cat. No. 302925)
AB_10644005 (BioLegend Cat. No. 302926)
- Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
- T cell costimulation
- CD80, CD86
- Cell Type
- B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
- Gene ID
- 940 View all products for this Gene ID
- View information about CD28 on UniProt.org
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