Brilliant Violet 421™ anti-human CD197 (CCR7) Antibody

Pricing & Availability
Clone
G043H7 (See other available formats)
Regulatory Status
RUO
Other Names
BLR2, CDw197, EBI1, CMKBR7
Isotype
Mouse IgG2a, κ
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Product Citations
publications
G043H7_BV421_1_101111
Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7/CD197 (clone G043H7) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • G043H7_BV421_1_101111
    Human peripheral blood lymphocytes were stained with CD3 FITC and CCR7/CD197 (clone G043H7) Brilliant Violet 421™ (top) or mouse IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • G043H7_BV421_2_101111
See Brilliant Violet 421™ spectral data View NHP data of the APC format.
Cat # Size Price Quantity Avail. Save
353207 25 tests £133
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353208 100 tests £253
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Description

CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CCR7-transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Product Citations
  1. Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
  2. Spandan V Shah et al. 2018. Cell reports. 25(10):2766-2774 . PubMed
  3. Barcelo H, et al. 2018. Curr Protoc Cytom. 84:e35. PubMed
  4. Marcandalli J et al. 2019. Cell. 176(6):1420-1431 . PubMed
  5. Darrah PA, et al. 2019. NPJ Vaccines. 4:21. PubMed
  6. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  7. Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
  8. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  9. Sparber F, et al. 2019. Cell Host Microbe. 25:389. PubMed
  10. Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed
  11. Nobs SP, et al. 2017. J Exp Med. 214:3015. PubMed
  12. Thyagarajan B, et al. 2018. J Immunol Methods. 463:61. PubMed
  13. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  14. Thompson EA, et al. 2019. Cell Rep. 28:1127. PubMed
  15. Azeem W, et al. 2020. Front Immunol. 11:438. PubMed
  16. Cassotta A, et al. 2019. Nat Med. 25:1402. PubMed
  17. Ols S, et al. 2020. Cell Reports. 30(12):3964-3971. PubMed
  18. Grifoni A, et al. 2020. Cell. 181(7):1489-1501.e15.. PubMed
  19. Neumann B, et al. 2015. J Leukoc Biol. 97:19. PubMed
  20. Gerna G, et al. 2015. J Gen Virol. 96:360. PubMed
  21. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  22. Wallin J, et al. 2016. Nat Commun. 7:12624. PubMed
  23. Geiger R, et al. 2016. Cell. 167:829-842. PubMed
  24. Benner M, et al. 2020. Cell Rep. 32:108204. PubMed
  25. Waddington KE, et al. 2020. Front Immunol. 1.51875. PubMed
  26. Cassotta A, et al. 2020. J Exp Med. 217:00:00. PubMed
  27. Eslamizar L, et al. 2021. NPJ Vaccines. 6:15. PubMed
  28. Pais Ferreira D, et al. 2020. Immunity. 53(5):985-1000.e11. PubMed
  29. Szabo PA, et al. 2021. Immunity. 54(4):797-814.e6. PubMed
  30. Mandolesi M, et al. 2021. Cell Reports Medicine. 2(4):100252. PubMed
  31. Ramaswamy A, et al. 2021. Immunity. 54(5):1083-1095.e7. PubMed
  32. Pan YG, et al. 2021. Immunity. 54(6):1245-1256.e5. PubMed
  33. Richert-Spuhler LE, et al. 2021. Cell Reports Medicine. 2(6):100322. PubMed
  34. Bziat V, et al. 2021. Cell. . PubMed
RRID
AB_10915137 (BioLegend Cat. No. 353207)
AB_11203894 (BioLegend Cat. No. 353208)

Antigen Details

Structure
Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
Distribution

T cells, B cells, NK, dendritic cells.

Function
The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
Ligand/Receptor
CCL19 and CCL21.
Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed

Gene ID
1236 View all products for this Gene ID
UniProt
View information about CD197 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 2    Revision Date: 04/08/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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