- MEM-55 (See other available formats)
- Regulatory Status
- V CD45.08
- Other Names
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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CD45RB is a 180-240 kD single chain type I membrane glycoprotein. It is an exon 5 splice variant of the tyrosine phosphatase CD45. The CD45RB isoform is expressed on thymocytes, T cell subsets, B cells, monocytes/macrophages, granulocytes, and dendritic cells. CD45RB enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines. The MEM-55 antibody recognizes a silaidase-sensitive epitope of CD45.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Human thymocytes and T lymphocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The MEM-55 antibody is specific for a glycosylated-CD45RB epitope that is highly expressed on memory B cells and plasmablasts but not on naïve B cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting, and immunohistochemical staining of formalin-fixed paraffin-embedded tissue sections.
(PubMed link indicates BioLegend citation)
- Horejsi V, et al. 1988. Folia Biol. 34:23.
- Bazil V, et al. 1989. Immunogenetics 29:202.
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Koethe S, et al. 2011. J. Leukoc. Biol. 90:5.
- Product Citations
AB_314807 (BioLegend Cat. No. 310204)
- Tyrosine phosphatases, type I transmembrane, 180-240 kD (exon 5 splicing of CD45 gene)
Thymocytes, T cell subset, B cells, monocytes/macrophages, granulocytes, dendritic cells
- Enhances TCR and BCR signaling
- Galectin-1, CD2, CD3, CD4
- Cell Type
- B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
- Gene ID
- 5788 View all products for this Gene ID
- View information about CD45RB on UniProt.org
Related Pages & Pathways
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other FormatsView All CD45RB Reagents Request Custom Conjugation
|PE anti-human CD45RB||MEM-55||FC|
|Purified anti-human CD45RB||MEM-55||FC,IHC-P,IP,WB|
|FITC anti-human CD45RB||MEM-55||FC|
|Alexa Fluor® 594 anti-human CD45RB||MEM-55||IHC-P|
|TotalSeq™-A0844 anti-human CD45RB||MEM-55||PG|
|TotalSeq™-C0844 anti-human CD45RB Antibody||MEM-55||PG|
|TotalSeq™-B0844 anti-human CD45RB||MEM-55||PG|
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