- UCHL1 (See other available formats)
- Regulatory Status
- IV N31
- Other Names
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD45RO is a 180 kD single chain membrane glycoprotein. It is a splice variant of tyrosine phosphatase CD45, lacking the A, B, and C determinants. The CD45RO isoform is expressed on activated and memory T cells, some B cell subsets, activated monocytes/macrophages, and granulocytes. CD45RO enhances both T cell receptor and B cell receptor signaling mediated activation. CD45 and its isoforms non-covalently associate with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1 and CD22. CD45 isoform expression can change in response to cytokines.Product Details
- Human, Chimpanzee, Cynomolgus, Common Marmoset
- Antibody Type
- Host Species
- IL-2 dependent T cell line, CA1
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- µg sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.2 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The UCHL1 antibody is commonly used in combination with antibodies against CD45RA to discern memory and naïve T cells. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections5 and formalin-fixed paraffin-embedded tissue sections4, Western blotting2, and immunoprecipitation3.
(PubMed link indicates BioLegend citation)
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (FC)
- Ishii T, et al. 2001. P. Natl. Acad. Sci. USA 98:12138. (WB)
- Ponsford M, et al. 2001. Clin. Exp. Immunol. 124:315. (IP)
- Yamada M, et al. 1996. Stroke 27:1155. (IHC)
- Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immunol. 5:430. (IHC)
- Baba N, et al. 2010. Int. Immunol. 22:237. PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Weiss L, et al. 2010. P. Natl. Acad. Sci. USA 107:10632. PubMed
- Wu YY, et al. 2007. Infect. Immun. 75:4357. PubMed
- Mozaffarian N, et al. 2008. Rheumatology 47:1335. PubMed
- Roque S, et al. 2007. J. Immunol. 178:8028. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Smith SH, et al. 1986. Immunology 58:63. (Immunogen)
- Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
- Product Citations
AB_314421 (BioLegend Cat. No. 304205)
AB_2564160 (BioLegend Cat. No. 304244)
AB_314422 (BioLegend Cat. No. 304206)
- Tyrosine phosphatases, type I transmembrane, 180 kD (isoform of CD45 containing none of the A, B, or C determinants)
Activated and memory T cells, B cell subsets, monocytes, macrophages, granulocytes
- Enhances TCR and BCR signaling
- Cell Type
- B cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol. 12:85.
- Gene ID
- 5788 View all products for this Gene ID
- View information about CD45RO on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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APC anti-human CD45RO
FITC anti-human CD45RO
PE anti-human CD45RO
PE/Cyanine5 anti-human CD45RO
Purified anti-human CD45RO
Alexa Fluor® 488 anti-human CD45RO
Pacific Blue™ anti-human CD45RO
Alexa Fluor® 700 anti-human CD45RO
Biotin anti-human CD45RO
Brilliant Violet 421™ anti-human CD45RO
PerCP/Cyanine5.5 anti-human CD45RO
Brilliant Violet 570™ anti-human CD45RO
Brilliant Violet 605™ anti-human CD45RO
APC/Cyanine7 anti-human CD45RO
PE/Cyanine7 anti-human CD45RO
Brilliant Violet 650™ anti-human CD45RO
Brilliant Violet 711™ anti-human CD45RO
Brilliant Violet 785™ anti-human CD45RO
Alexa Fluor® 594 anti-human CD45RO
Purified anti-human CD45RO (Maxpar® Ready)
Brilliant Violet 510™ anti-human CD45RO
PE/Dazzle™ 594 anti-human CD45RO
APC/Fire™ 750 anti-human CD45RO
PerCP anti-human CD45RO
APC anti-human CD45RO
TotalSeq™-A0087 anti-human CD45RO
TotalSeq™-B0087 anti-human CD45RO
TotalSeq™-C0087 anti-human CD45RO
Brilliant Violet 750™ anti-human CD45RO
PE/Fire™ 640 anti-human CD45RO
TotalSeq™-D0087 anti-human CD45RO
Spark Violet™ 423 anti-human CD45RO
GMP APC anti-human CD45RO