Biotin anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody

Pricing & Availability
Clone
m2B4 (B6)458.1 (See other available formats)
Regulatory Status
RUO
Other Names
2B4 B6 Alloantigen
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_m2B4_Biotin_060309.jpg
C57BL/6 splenocytes stained with m2B4 (B6) 458.1 biotin (upper panel) or mouse IgG1 isotype control biotin (lower panel) followed by Sav-PE and anti-mouse CD49b DX5 APC
  • A_m2B4_Biotin_060309.jpg
    C57BL/6 splenocytes stained with m2B4 (B6) 458.1 biotin (upper panel) or mouse IgG1 isotype control biotin (lower panel) followed by Sav-PE and anti-mouse CD49b DX5 APC
  • B_m2B4_Biotin_060309.jpg
Cat # Size Price Quantity Check Availability Save
133505 50 µg £48.00
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133506 200 µg £120.00
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Description

Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
m2B4-Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.

Application References

(PubMed link indicates BioLegend citation)
  1. Lee KM, et al. 2006. Blood. 107:3181.
  2. Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
  3. Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
  4. de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed
Product Citations
  1. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  2. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
RRID
AB_1626229 (BioLegend Cat. No. 133505)
AB_1626222 (BioLegend Cat. No. 133506)

Antigen Details

Structure
A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
Distribution

All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.

Function
Mediate non-MHC-restricted cytotoxicity.
Ligand/Receptor
CD48
Cell Type
Dendritic cells, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
18106 View all products for this Gene ID
UniProt
View information about CD244.2 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 03/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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