Purified anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody

Pricing & Availability
m2B4 (B6)458.1 (See other available formats)
Regulatory Status
Other Names
2B4 B6 Alloantigen
Mouse IgG1, κ
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Product Citations
C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1.1 APC
  • m2B4_Pure_050109.jpg
    C57BL/6 splenocytes stained with m2B4(B6)458.1 FITC and NK-1.1 APC
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133501 50 µg £38.00
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Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IP, IHC, ELISA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.

Application References

(PubMed link indicates BioLegend citation)
  1. Lee KM, et al. 2006. Blood. 107:3181.
  2. Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
  3. Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
  4. de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed
Product Citations
  1. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
AB_1595628 (BioLegend Cat. No. 133501)

Antigen Details

A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).

All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.

Mediate non-MHC-restricted cytotoxicity.
Cell Type
Dendritic cells, NK cells, NKT cells, T cells
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
18106 View all products for this Gene ID
View information about CD244.2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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