APC/Fire™ 750 anti-mouse CD244.2 (2B4 B6 Alloantigen) Antibody

Pricing & Availability
Clone
m2B4 (B6)458.1 (See other available formats)
Other Names
2B4 B6 Alloantigen
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
m2B4-B6-458dot1_APCFire750_CD244dot2_Antibody_060319
C57BL/6 splenocytes were stained with CD49b PE and CD244.2 (2B4 B6 Alloantigen) (clone m2B4 (B6)458.1) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).
  • m2B4-B6-458dot1_APCFire750_CD244dot2_Antibody_060319
    C57BL/6 splenocytes were stained with CD49b PE and CD244.2 (2B4 B6 Alloantigen) (clone m2B4 (B6)458.1) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
133515 25 µg £104
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133516 100 µg £225
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Description

Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non- MHC-restricted cytotoxicity. It is expressed on all natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes, including dendritic epidermal T cells. There are at least two isoforms of CD244.2 proteins that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner, while the short form functions in an activating manner. The ligand of CD244 is CD48, which is expressed on all hematopoietic cells. It was reported that CD244 interaction with CD48 is essential for IL-2-driven expansion and activation of murine NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
m2B4-Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Application Notes

The 2B4 antibody reacts with CD244.2, the 2B4 allloantigen which is expressed on C57BL/6 mice, but not on BALB/c mice.

Application References

(PubMed link indicates BioLegend citation)
  1. Lee KM, et al. 2006. Blood. 107:3181.
  2. Schatzle JD, et al. 1999. P. Natl Acad Sci USA. 96:3870.
  3. Stepp SE, et al. 1999. Eur. J. Immunol. 29:2392.
  4. de la Luz Sierra M, et al. 2010. Blood. 115:3970. PubMed

Antigen Details

Structure
A member of the CD2 subset of the immunoglobulin superfamily (CD2 IgSF).
Distribution

All natural killer (NK) cells, IL-2 activated NK (LAK) cells, NKT cells, and a subset of T lymphocytes.

Function
Mediate non-MHC-restricted cytotoxicity.
Ligand/Receptor
CD48
Cell Type
Dendritic cells, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Brown MH, et al. 1998. J. Exp. Med. 188:2083.
2. Davis SJ, et al. 1996. Immunol Today. 17:177.
3. Kumaresan PR, et al. 2000. Immunogenetics. 51:306.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
18106 View all products for this Gene ID
UniProt
View information about CD244.2 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 06/03/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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