APC anti-BrdU Antibody

Pricing & Availability
Clone
Bu20a (See other available formats)
Regulatory Status
RUO
Other Names
Bromodeoxyuridine
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
Bu20a_APC_051311
Human T lymphoblastic leukemia cell line, Hut-78, was pulsed with BrdU for 1 hour, fixed and permeabilized with 70% cold ethanol, and then stained with anti-BrdU (clone Bu20a) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram) by following BioLegend BrdU staining protocol.
  • Bu20a_APC_051311
    Human T lymphoblastic leukemia cell line, Hut-78, was pulsed with BrdU for 1 hour, fixed and permeabilized with 70% cold ethanol, and then stained with anti-BrdU (clone Bu20a) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram) by following BioLegend BrdU staining protocol.
See APC spectral data
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339807 25 tests £75.00
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339808 100 tests £195.00
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Description

BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Magaud JP, et al. 1989. J. Histochem. Cytochem. 37:1517.
  2. Ejassi-Lassallette A, et al. 2011. Mol. Biol. Cell 22:245. PubMed
  3. Tan YS, et al. 2014. PLoS One. 9:111777. PubMed
Product Citations
  1. Ferrandino F, et al. 2018. Oncogene. 37:6285. PubMed
  2. Paschalis EI, et al. 2018. Proc Natl Acad Sci U S A. 115:E11359. PubMed
  3. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  4. Sundaravinayagam D et al. 2019. Cell Rep. 28(6):1389-1399 . PubMed
  5. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  6. Matsuoka S, et al. 2019. Haematologica. 105:226. PubMed
  7. Jimenez RV, et al. 2019. Front Immunol. 1.932638889. PubMed
  8. Morrison K, et al. 2019. Cell Rep. 29:2961. PubMed
  9. Joshi JC, et al. 2020. Cell Rep. 30:4096. PubMed
  10. Xing M, et al. 2020. Molecular Cell. 78(4):714-724. PubMed
  11. Tan Y, et al. 2014. PLoS One. 9:111777. PubMed
  12. Kamei A, et al. 2016. Proc Natl Acad Sci U S A. 113: E6153 - E6161. PubMed
  13. Branigan TB, et al. 2021. Cell Reports. 34(9):108808. PubMed
RRID
AB_10900446 (BioLegend Cat. No. 339807)
AB_10895898 (BioLegend Cat. No. 339808)

Antigen Details

Structure
Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution
Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Function
Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. P. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.

Gene ID
NA
UniProt
View information about BrdU on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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