PE anti-BrdU Antibody

Pricing & Availability
Bu20a (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Brdu-incorporated Hut-78 cells stained with BU20a PE
  • BU20A_PE_061109.jpg
    Brdu-incorporated Hut-78 cells stained with BU20a PE
See PE spectral data
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339811 25 tests £72.00
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339812 100 tests £188.00
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BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Magaud JP, et al. 1989. J. Histochem. Cytochem. 37:1517.
  2. Hosoya T, et al. 2009. J. Exp Med. 206:2987. PubMed
  3. Zhang Y, et al. 2013. J. Immunol. 190:4725. PubMed.
Product Citations
  1. Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed
  2. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  3. Liu G, et al. 2019. Int J Biol Sci. 15:1383. PubMed
  4. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  5. Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed
  6. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  7. Hosoya T, et al. 2009. J Exp Med. 206:2987. PubMed
  8. Zhang Y, et al. 2013. J Immunol. 900:4725. PubMed
  9. Frei A, et al. 2016. Nat Methods. 10.1038/nmeth.3742. PubMed
  10. Kang DW, et al. 2020. J Pathol. 252:304. PubMed
AB_1626188 (BioLegend Cat. No. 339811)
AB_1626186 (BioLegend Cat. No. 339812)

Antigen Details

Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family
Nuclear Markers
Antigen References

1. Gratzner HG. 1982. Science 218:474.
2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573.
3. Dolbeare F, et al. 1985. Cytometry 6:521.

Gene ID
View information about BrdU on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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