PerCP/Cyanine5.5 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_PerCPCyanine55_F4-80_Antibody_010319
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) PerCP/Cyanine5.5 (left) or rat IgG2a, ? isotype control PerCP/Cyanine5.5 (right).
  • BM8_PerCPCyanine55_F4-80_Antibody_010319
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) PerCP/Cyanine5.5 (left) or rat IgG2a, ? isotype control PerCP/Cyanine5.5 (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
123127 25 µg 104€
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123128 100 µg 244€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Song P, et al. 2018. Int J Mol Med. 41:935. PubMed
  2. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  3. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  4. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  5. Tyagi AM et al. 2018. Immunity. 49(6):1116-1131 . PubMed
  6. Macdougall CE et al. 2018. Cell metabolism. 27(3):588-601 . PubMed
  7. Duan H et al. 2018. Immunity. 49(2):301-311 . PubMed
  8. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  9. Boehm DT, et al. 2018. Infect Immun. 86:. PubMed
  10. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  11. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  12. Wang F, et al. 2018. Carcinogenesis. 39:889. PubMed
  13. Thompson PJ et al. 2019. Cell metabolism. 29(5):1045-1060 . PubMed
  14. Li Q, et al. 2019. Neuron. 101:207. PubMed
  15. Tong CF, et al. 2018. Am J Physiol Cell Physiol. 315:C409. PubMed
  16. Saint Fleur–Lominy S, et al. 2018. Cell Rep. 24:3045. PubMed
  17. Wu M, et al. 2020. Mucosal Immunol. 13:47. PubMed
  18. Buerfent BC, et al. 2019. GMS Infect Dis. 7:Doc04. PubMed
  19. Kunishita Y, et al. 2020. Front Immunol. 11:98. PubMed
  20. Mul Fedele ML, et al. 2020. Front Cell Infect Microbiol. 10:100. PubMed
  21. Si Y, et al. 2018. J Control Release. 282:120. PubMed
  22. Sztwiertnia I, et al. 2020. PLoS One. 15:e0233789. PubMed
  23. Brykczynska U, et al. 2020. Cell Reports. 30(5):1627-1643.e7.. PubMed
  24. Jaitin DA, et al. 2020. Cell. 178(3):686-698.e14.. PubMed
  25. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  26. Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
  27. Zellweger R, et al. 2010. Cell Host Microbe. 18:128. PubMed
  28. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  29. Kaku H, et al. 2014. J Immunol. 193:5904. PubMed
  30. Chou H, et al. 2015. Proc Natl Acad Sci U S A. 112:2175. PubMed
  31. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  32. Chen Z, et al. 2015. Am J Physiol Endocrinol Metab. 308:460. PubMed
  33. Calderon B, et al. 2015. J Exp Med. 212: 1497-1512. PubMed
  34. Meunier I, et al. 2015. PLoS One. 10: 0138055. PubMed
  35. Traboulsi H, et al. 2015. Antimicrob Agents Chemother. 59: 6317. PubMed
  36. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  37. Lin D, et al. 2016. Cancer Res . 76: 3179 - 3188. PubMed
  38. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  39. RY H, et al. 2016. Oncoimmunology. 6:e1249561. PubMed
  40. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  41. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  42. Si Y, et al. 2020. Sci Adv. 6:eaba0995. PubMed
  43. Zhang X, et al. 2020. Arterioscler Thromb Vasc Biol. 40:597. PubMed
  44. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  45. Balog S, et al. 2020. Hepatology. 71:291. PubMed
  46. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  47. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
  48. Chen X, et al. 2021. Immunity. 54(2):324-339.e8. PubMed
  49. Garg M, et al. 2021. Cell Reports. 34(6):108736. PubMed
  50. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
RRID
AB_893496 (BioLegend Cat. No. 123127)
AB_893484 (BioLegend Cat. No. 123128)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 01.03.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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