TruStain FcX™ (anti-mouse CD16/32) Antibody

Pricing & Availability
Clone
93 (See other available formats)
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
Ave. Rating
5 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
101319 50 µg 36€
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101320 500 µg 156€
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Description

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.

Application References

(PubMed link indicates BioLegend citation)
  1. Oliver AM, et al. 1999. Hybridoma 18:113.
  2. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed
  3. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
  4. Clements JL, et al. 2006. J. Immunol. 177:905. PubMed
  5. Flores M, et al. 2008. FASEB J. 22:3661. PubMed
  6. Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed
  7. Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed
  8. Lewis ND, et al. 2013. J. Immunol. 190:3533. PubMed
  9. Bonne-Annee S, et al. 2013. Infect Immun. 81:3346. PubMed
  10. Parlane NA, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  11. Jarajapu YP, et al. 2014. PLoS One. 9:93965. PubMed
  12. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
  13. Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
  14. Gillis TP, et al. 2014. Infect Immun. 82:3900. PubMed
  15. Minkah N, et al. 2015. J Virol. 89:3366. PubMed
Product Citations
  1. Ge X, et al. 2010. J Immunol. 185:1205. PubMed
  2. Kenyon K, et al. 2011. J Immunol. 187:2101. PubMed
  3. Gerald N, et al. 2011. PLoS One. 6:e24398. PubMed
  4. Maseda D, et al. 2012. J Immunol. 188:1036. PubMed
  5. Lewis N, et al. 2013. J Immunol. 190:3533. PubMed
  6. Bonne-Année S, et al. 2013. Infect Immun . 61:3346. PubMed
  7. Parlane N, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  8. Jarajapu Y, et al. 2014. PLoS One. 9:93965. PubMed
  9. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  10. Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
  11. Gillis T, et al. 2014. Infect Immun . 82:3900. PubMed
  12. Minkah N, et al. 2015. J Virol. 89:3366. PubMed
  13. Cieniewicz B, et al. 2015. J Virol. 89: 6562 - 6574. PubMed
  14. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  15. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  16. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  17. Lammers K, et al. 2015. PLoS One. 10: 0138338. PubMed
  18. Wang L, et al. 2015. Cancer Immunol Res. 3: 1030-1041. PubMed
  19. Kunisawa J, et al. 2015. Immunity. 1: 122-131. PubMed
  20. Ali A, et al. 2015. J Infect Dis. 212: 1308 - 1316. PubMed
  21. Frost E, et al. 2015. J Immunol. 195: 3520 - 3524. PubMed
  22. Huang W, et al. 2015. J Allergy Clin Immunol. . PubMed
  23. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  24. Yang Y, et al. 2016. Nucleic Acids Res. 44: 4174 - 4188. PubMed
  25. Tsaousi A, et al. 2016. PLoS One. 11: 0148873. PubMed
  26. Li Z, et al. 2016. Sci Rep. 6:22143. PubMed
  27. Ferretti M, et al. 2016. Brain Behav Immun. 54: 211-225. PubMed
  28. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  29. Jayaraman P, et al. 2016. PLoS One. 12: 1005490. PubMed
  30. Lykken J, et al. 2016. Blood. 127: 1886-1895. PubMed
  31. Li Z, et al. 2016. Nat Commun. 7: 11394. PubMed
  32. Nakagawa A, et al. 2016. Sci Rep. 6: 25009. PubMed
  33. Gupta P, et al. 2016. PLoS One. 11: 0154725. PubMed
  34. Provine N, et al. 2016. J Virol. 90: 4278 - 4288. PubMed
  35. Samusik N, et al. 2016. Nat Methods. 13: 493-496. PubMed
  36. Vasam G, Joshi S, Jarajapu Y 2016. Sci Rep. 6: 26131. PubMed
  37. Asson-Batres M, et al. 2016. Am J Physiol Heart Circ Physiol. 310: 1773 - 1789. PubMed
  38. Buonomo E, et al. 2016. Cell Rep. 16: 432-443. PubMed
  39. Johnson A, et al. 2016. Mol Metab. 5:506-526. PubMed
  40. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  41. Li D, et al. 2016. Sci Rep. 6:31002. PubMed
  42. Reddy S, et al. 2016. MBio. 7: e00723-16. PubMed
  43. Hey Y, O’Neill H 2016. PLoS One. 11: 0162358. PubMed
  44. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  45. Belmonte L, et al. 2016. Sci Rep. 6:35813. PubMed
  46. Tam H, et al. 2016. Proc Natl Acad Sci U S A. 113: E6639 - E6648. PubMed
  47. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  48. Galand C, et al. 2016. J Allergy Clin Immunol. 138:1356-1366. PubMed
  49. Reyes J, et al. 2016. Infect Immun . 84(12):3471-3483. PubMed
  50. Ju X, et al. 2016. J Immunol. 197(12):4613-4625. PubMed
  51. Bracamonte-Baran W, et al. 2017. Proc Natl Acad Sci U S A. 114(5):1099-1104. PubMed
  52. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  53. Cederquist C, et al. 2017. Mol Metab. 6(1):125-137. PubMed
  54. Lynch T, et al. 2017. J Mol Cell Cardiol. 102:83-93. PubMed
  55. Chiang N, et al. 2017. J Immunol. 198(2):842-851. PubMed
  56. Cortez-Toledo O, et al. 2017. PLoS One. 12(2):e0171268. PubMed
  57. Cuccarese M, et al. 2017. Nat Commun. 8:14293. PubMed
  58. Beug S, et al. 2017. Nat Commun. 10.1038/ncomms14278. PubMed
  59. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  60. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  61. Watanabe K, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006513. PubMed
  62. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  63. Zhao J, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.149. PubMed
  64. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  65. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  66. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  67. Chen W,et al. 2017. Sci Rep.. 10.1038/s41598-017-13570-2. PubMed
  68. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  69. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  70. Wartewig T, et al. 2017. Nature. . 10.1038/nature24649. PubMed
  71. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  72. Chen D, et al. 2018. Nat Commun. 9:873. PubMed
  73. Abraham V, et al. 2018. Int J Oncol. 53:488. PubMed
  74. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  75. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  76. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  77. Zöller T, et al. 2018. Int J Mol Sci. 19:E706. PubMed
  78. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  79. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  80. van Vloten JP, et al. 2019. Mol Ther Methods Clin Dev. 13:154. PubMed
  81. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  82. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  83. Yrlid U, et al. 2019. J Leukoc Biol. 105:195. PubMed
  84. Wong EL, et al. 2018. Brain Behav Immun. 67:257. PubMed
  85. Schappe MS, et al. 2018. Immunity. 48:59. PubMed
  86. Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed
  87. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  88. Koizumi SI, et al. 2018. Nat Commun. 9:5344. PubMed
  89. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  90. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  91. Manglani M, et al. 2018. Curr Protoc Immunol. 121:e44. PubMed
  92. Dillon MT, et al. 2019. Clin Cancer Res. 25:3392. PubMed
  93. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  94. Agelidis A, et al. 2019. Front Immunol. 10:500. PubMed
  95. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  96. Mitchell LA, et al. 2019. Oncotarget. 10:2252. PubMed
  97. Tsuchiya K, et al. 2019. Nat Commun. 10:2091. PubMed
  98. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  99. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  100. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  101. Maximov V, et al. 2019. Nat Commun. 10:2410. PubMed
  102. Eslani M, et al. 2018. Stem Cells. 36:775. PubMed
  103. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  104. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  105. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  106. Axelrod HD, et al. 2019. Mol Cancer Res. 17:356. PubMed
  107. Li B, et al. 2018. Cancer Immunol Res. 6:539. PubMed
  108. Tanigawa Y, et al. 2019. Nat Commun. 10:4064. PubMed
  109. Haupt F, et al. 2019. Sci Rep. 9:9798. PubMed
  110. Gomez D, et al. 2018. Nat Med. 24:1418. PubMed
  111. Sweere JM, et al. 2019. Science. 363. PubMed
  112. Wirsching HG, et al. 2019. JCI Insight. 4. PubMed
  113. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  114. Wang Y, et al. 2019. Front Cell Infect Microbiol. 9:286. PubMed
  115. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  116. Wu Y, et al. 2019. Nat Med. 25:776. PubMed
  117. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  118. Acker KP, et al. 2019. iScience. 19:281. PubMed
  119. Kwee BJ, et al. 2019. Sci Adv. 5:eaav6313. PubMed
  120. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  121. Hickman SE, et al. 2019. Methods Mol Biol. 2034:305. PubMed
  122. Engler JB, et al. 2019. J Immunol. 203:1743. PubMed
  123. Fox JJ, et al. 2019. Am J Clin Exp Urol. 7:281. PubMed
  124. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  125. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  126. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  127. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  128. Sokol CL et al. 2018. Immunity. 49(3):449-463 . PubMed
  129. Jacobson A et al. 2018. Cell host & microbe. 24(2):296-307 . PubMed
  130. Miura Y et al. 2018. eLife. 7 pii: e36572. PubMed
  131. Divakaruni AS et al. 2018. Cell metabolism. 28(3):490-503 . PubMed
  132. Moor AE et al. 2018. Cell. 175(4):1156-1167 . PubMed
  133. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  134. Contijoch EJ et al. 2019. eLife. 8 pii: e40553. PubMed
  135. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  136. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  137. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  138. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  139. Crowell PD et al. 2019. Cell Rep. 28(6):1499-1510 . PubMed
  140. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  141. Tran NT et al. 2019. Cell Rep. 28(13):3510-3522 . PubMed
  142. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  143. Limon JJ et al. 2019. Cell host & microbe. 25(3):377-388 . PubMed
  144. Lubkin A et al. 2019. Cell host & microbe. 25(3):463-470 . PubMed
  145. Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
  146. Green DP et al. 2019. Neuron. 101(3):412-420 . PubMed
  147. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  148. Delgado‐Benito V et al. 2018. Molecular cell. 72(4):636-649 . PubMed
  149. Wang J et al. 2018. Cell. 176(1-2):334-347 . PubMed
  150. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  151. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  152. Liu H et al. 2017. Cell host & microbe. 22(5):653-666 . PubMed
  153. Zhu Y et al. 2017. The Journal of Neuroscience. 37(9):2362-2376 . PubMed
  154. Leanza L et al. 2017. Cancer cell. 31(4):516-531 . PubMed
  155. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  156. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  157. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  158. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
  159. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  160. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  161. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  162. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  163. Chen L, et al. 2019. Nat Genet. 51:777. PubMed
  164. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  165. Chan LC, et al. 2019. J Clin Invest. 129:3324. PubMed
  166. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  167. Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed
  168. Nambiar DK, et al. 2019. J Clin Invest. 129:5553. PubMed
  169. Wang C, et al. 2020. Mucosal Immunol. 13:22. PubMed
  170. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  171. Panagi I, et al. 2020. Cell Host Microbe. 27:41:00. PubMed
  172. Wang G, et al. 2020. Nat Commun. 0.611111111. PubMed
  173. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  174. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  175. Brunner JS, et al. 2020. Nat Commun. 0.757638889. PubMed
RRID
AB_1574973 (BioLegend Cat. No. 101319)
AB_1574975 (BioLegend Cat. No. 101320)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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