PE/Cyanine7 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
BM8_PECy7_122607
Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with BM8 PE/Cyanine7
  • BM8_PECy7_122607
    Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with BM8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
123113 25 µg 84€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
123114 100 µg 243€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fujikura D, et al. 2013. PLoS One. 8:e55321. PubMed
  2. Song M, et al. 2013. Am J Pathol. 182:1308. PubMed
  3. Sharif O, et al. 2013. J Immunol. 190:5640. PubMed
  4. Chan M, et al. 2013. J Cell Sci. 126:3948. PubMed
  5. Patnode M, et al. 2013. J Biol Chem. 288:26533. PubMed
  6. Kong P, et al. 2013. Am J Physiol Heart Circ Physiol. 305:1363. PubMed
  7. Saxena A, et al. 2013. J Immunol. 191:4838. PubMed
  8. Weber G, et al. 2014. J Exp Med. 211:1243. PubMed
  9. Denis C, et al. 2014. Nucleic Acids Res. 42:7370. PubMed
  10. Tay S, et al. 2014. J Immunol. 193:2087. PubMed
  11. Chiu Y, et al. 2014. J Immunol. 193:2207. PubMed
  12. Mojumdar K, et al. 2014. EMBO Mol Med. 6:1476. PubMed
  13. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  14. Giordano C, et al. 2014. Hum Mol Genet. . PubMed
  15. Hamilton J, et al. 2015. J Immunol. 194:5022. PubMed
  16. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  17. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  18. Kim P, et al. 2015. Infect Immun . 83: 2992 - 3002. PubMed
  19. Sive J, et al. 2015. Leukemia. . PubMed
  20. Tozuka M, et al. 2016. J Dermatol Sci. 82: 38-45. PubMed
  21. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  22. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  23. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  24. Mangum L, et al. 2016. Toxicol Sci. 150: 169 - 177. PubMed
  25. Jondle C, et al. 2016. J Immunol. 196: 3088-3096. PubMed
  26. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  27. INAGAWA H, et al. 2016. In Vivo. 30: 205 - 211. PubMed
  28. Kwon H, Kim S 2016. Cell Death Dis. 7:22285. PubMed
  29. Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
  30. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  31. Coppo M, et al. 2016. Nat Commun. 7:12254. PubMed
  32. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  33. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  34. Allegrezza M, et al. 2016. Cancer Res . 76: 6253 - 6265. PubMed
  35. Harvey RE et al. 2017. Endocrinology. 158(7):2179-2189 . PubMed
  36. Alonso-Herranz L, et al. 2020. Elife. 9:00. PubMed
  37. Wanner-Seleznik GM, et al. 2020. Immunohorizons. 0.644444444. PubMed
  38. Heim CE, et al. 2020. Nature Microbiology. 5(10):1271-1284. PubMed
  39. Ren Y, et al. 2020. Stem Cell Res Ther. 0.743055556. PubMed
  40. Senatus L, et al. 2020. JCI Insight. 5:00. PubMed
  41. Urbanska AM, et al. 2020. Cancer Biother Radiopharm. 35:459. PubMed
  42. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  43. Li J, et al. 2020. FASEB J. 34:3091. PubMed
  44. Tripathi H, et al. 2020. Stem Cell Rev Rep. 0.953472222. PubMed
  45. Cornax I, et al. 2021. Microorganisms. 9:00. PubMed
  46. Sheng J, et al. 2021. eLife. 10:00. PubMed
  47. Szafran BN, et al. 2021. Chemical Research in Toxicology. 34(6):1556-1571. PubMed
  48. Hall JA, et al. 2020. Cell Metabolism. 32(4):665-675.e6. PubMed
  49. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  50. Yun H, et al. 2020. Immunity. 53(5):1050-1062.e5. PubMed
  51. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  52. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  53. Yamakawa D, et al. 2021. Cell Reports. 34(10):108817. PubMed
  54. Dallari S, et al. 2021. Cell Host Microbe. 29(6):1014-1029.e8. PubMed
  55. Sangaletti S, et al. 2021. iScience. 24(6):102510. PubMed
  56. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  57. Bae S, et al. 2021. Cell Reports. 35(11):109264. PubMed
  58. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  59. Oertel S, et al. 2017. Cell Mol Life Sci. 10.1007/s00018-017-2518-9. PubMed
  60. Li S, et al. 2017. Applied Materials & Interfaces. 10.1021/acsami.7b03160. PubMed
  61. Shimoda A, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.07.126. PubMed
  62. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
  63. Efremova M, et al. 2018. Nat Commun. 9:32. PubMed
  64. Chu LH, et al. 2018. Nat Commun. 9:996. PubMed
  65. Clemente C, et al. 2018. Nat Commun. 9:910. PubMed
  66. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  67. Youssif C, et al. 2018. EMBO Mol Med. 10:e8403. PubMed
  68. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  69. Xu X, et al. 2018. Cell. 175:1336. PubMed
  70. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  71. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
  72. Michela Miani et al. 2018. Cell metabolism. 28(4):557-572 . PubMed
  73. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  74. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  75. Frank AC, et al. 2019. Nat Commun. 10:1135. PubMed
  76. Sadtler K, et al. 2017. Tissue Eng Part A. 23:1044. PubMed
  77. Petriello MC, et al. 2018. Toxicol Sci. 162:548. PubMed
  78. Fachi JL et al. 2019. Cell reports. 27(3):750-761 . PubMed
  79. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  80. Shikatani EA et al. 2019. Cell Rep. 27(8):2304-2312 . PubMed
  81. Li C, et al. 2018. Cell. 174:285. PubMed
  82. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  83. Maston LD, et al. 2017. Am J Physiol Lung Cell Mol Physiol. 312:L609. PubMed
  84. Luo W, et al. 2019. Mol Med Rep. 20:1007. PubMed
  85. Koh EH, et al. 2018. Diabetes. 67(12). PubMed
  86. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  87. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  88. Ponzetta A, et al. 2020. Cell. 178(2):346-360.e24.. PubMed
  89. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  90. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  91. Xiong X et al. 2019. Mol Cell. 75(3):644-660 . PubMed
  92. Zhang S, et al. 2019. Cell Metab. 29:443. PubMed
  93. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  94. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  95. Nabet BY et al. 2017. Cell. 170(2):352-366 . PubMed
  96. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  97. Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
  98. Nalbandian A, et al. 2017. Inflammation. 40:21:00. PubMed
  99. Muchenditsi A, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 313:G39. PubMed
  100. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  101. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
  102. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  103. Zhao Y, et al. 2020. Immunity. 51(6):1059-1073.e9.. PubMed
  104. Shang W, et al. 2019. J Diabetes Res. 2019:5641271. PubMed
  105. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  106. Chou YJ, et al. 2020. Sci Rep. 10:8422. PubMed
  107. Yamada KJ, et al. 2020. PLoS Pathog. 16:e1008354. PubMed
  108. Currier MA, et al. 2017. Oncotarget. 8:17412. PubMed
  109. Terashima Y, et al. 2020. Nat Commun. 11:609. PubMed
  110. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  111. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  112. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  113. Zheng Z, et al. 2020. Cell Death Dis. 0.684722222. PubMed
  114. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
  115. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  116. Cai B, et al. 2020. Cell Metabolism. 31(2):406-421.e7.. PubMed
  117. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  118. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  119. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  120. Quenum Zangbede FO, et al. 2018. J Neurosci. 38:6737. PubMed
  121. Torroella-Kouri M, et al. 2009. Cancer Res. 69:4800. PubMed
RRID
AB_893490 (BioLegend Cat. No. 123113)
AB_893478 (BioLegend Cat. No. 123114)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.25.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Brilliant Violet 605™ anti-mouse F4/80

  • Purified anti-mouse F4/80

  • Biotin anti-mouse F4/80

  • FITC anti-mouse F4/80

  • PE anti-mouse F4/80

  • PE/Cyanine5 anti-mouse F4/80

  • PE/Cyanine7 anti-mouse F4/80

  • APC anti-mouse F4/80

  • APC/Cyanine7 anti-mouse F4/80

  • Alexa Fluor® 488 anti-mouse F4/80

  • Alexa Fluor® 647 anti-mouse F4/80

  • Pacific Blue™ anti-mouse F4/80

  • PerCP anti-mouse F4/80

  • PerCP/Cyanine5.5 anti-mouse F4/80

  • Alexa Fluor® 700 anti-mouse F4/80

  • Brilliant Violet 421™ anti-mouse F4/80

  • Brilliant Violet 510™ anti-mouse F4/80

  • Alexa Fluor® 594 anti-mouse F4/80

  • Brilliant Violet 785™ anti-mouse F4/80

  • Purified anti-mouse F4/80 (Maxpar® Ready)

  • PE/Dazzle™ 594 anti-mouse F4/80

  • Brilliant Violet 650™ anti-mouse F4/80

  • Brilliant Violet 711™ anti-mouse F4/80

  • APC/Fire™ 750 anti-mouse F4/80

  • TotalSeq™-A0114 anti-mouse F4/80

  • TotalSeq™-B0114 anti-mouse F4/80

  • TotalSeq™-C0114 anti-mouse F4/80

  • Spark YG™ 570 anti-mouse F4/80

  • KIRAVIA Blue 520™ anti-mouse F4/80

  • Ultra-LEAF™ Purified anti-mouse F4/80

  • APC/Fire™ 810 anti-mouse F4/80

  • Spark NIR™ 685 anti-mouse F4/80

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account