Brilliant Violet 605™ anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_BV605_F480_Antibody_FC_071013
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
  • BM8_BV605_F480_Antibody_FC_071013
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with F4/80 (clone BM8) Brilliant Violet 605™ (filled histogram) or rat IgG2a, κ Brilliant Violet 605™ isotype control (open histogram).
Compare all formats See Brilliant Violet 605™ spectral data
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123133 125 µL 181€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Vasanthakumar A, et al. 2020. Nature. 579:581. PubMed
  2. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  3. Schmid MC, et al. 2018. Nat Commun. 9:5379. PubMed
  4. Kim YS, et al. 2021. Cell Death Dis. 12:307. PubMed
  5. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  6. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  7. Vicetti Miguel R, et al. 2016. PLoS One. 11: 0162445. PubMed
  8. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  9. Salas–Pérdomo A, et al. 2019. Sci Rep. 9:8309. PubMed
  10. Karachi A, et al. 2019. Neuro Oncol. 1.381944444. PubMed
  11. Cunningham C, et al. 2016. PLoS Pathog. 12: 1005356. PubMed
  12. Korangath P, et al. 2020. Bio Protoc. 10:e3822. PubMed
  13. Pandey V, et al. 2021. eLife. 0.416666666666667. PubMed
  14. Chen L, et al. 2021. Medicines (Basel). 8:. PubMed
  15. Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed
  16. Ayaub E,et al. 2017. Sci Rep.. 10.1038/s41598-017-13511-z. PubMed
  17. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  18. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  19. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  20. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  21. Ayaub EA, et al. 2019. Immunol Cell Biol. 97:203. PubMed
  22. Penkert RR, et al. 2020. Obesity (Silver Spring). 1631:28. PubMed
  23. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  24. Qi F, et al. 2019. Front Microbiol. 2.140277778. PubMed
  25. Rosser EC, et al. 2020. Cell Metabolism. 31(4):837-851. PubMed
  26. Akoolo L, et al. 2021. Cell Microbiol. 23:e13350. PubMed
  27. Jablonski K, et al. 2015. PLoS One. 10: 0145342. PubMed
  28. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  29. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
  30. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  31. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  32. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  33. Kastenschmidt JM, et al. 2021. Cell Rep. 35:108997. PubMed
  34. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
RRID
AB_2562305 (BioLegend Cat. No. 123133)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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