Alexa Fluor® 700 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_Alx700_042110
Thioglycolate-elicited Balb/c peritoneal macrophages stained with BM8 Alexa Fluor® 700
  • BM8_Alx700_042110
    Thioglycolate-elicited Balb/c peritoneal macrophages stained with BM8 Alexa Fluor® 700
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123129 25 µg 115€
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123130 100 µg 252€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  3. Domingues CS, et al. 2020. Front Immunol. 11:566476. PubMed
  4. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  5. Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
  6. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  7. Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
  8. Fujita Y et al. 2018. Cell reports. 24(12):3296-3311 . PubMed
  9. Casadesús AV, et al. 2020. Oncoimmunology. 9:1770565. PubMed
  10. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  11. Mirando AC, et al. 2020. Oncoimmunology. 9:1760685. PubMed
  12. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  13. Redelinghuys P, et al. 2016. Ann Rheum Dis. 75: 1386 - 1391. PubMed
  14. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  15. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  16. Jonas B, et al. 2016. PLoS One. 11: 0159189. PubMed
  17. Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
  18. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  19. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  20. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  21. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  22. Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed
  23. Liu Y, et al. 2019. Sci Rep. 9:18970. PubMed
  24. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  25. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  26. Barsoumian HB, et al. 2022. Cancers (Basel). 14:. PubMed
  27. Smith LK, et al. 2021. Elife. 10:. PubMed
  28. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  29. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
RRID
AB_2277848 (BioLegend Cat. No. 123129)
AB_2293450 (BioLegend Cat. No. 123130)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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