Alexa Fluor® 700 anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
53-6dot7_Alx700_041106
C57BL/6 mouse splenocytes stained with CD8 (clone 53-6.7) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
  • 53-6dot7_Alx700_041106
    C57BL/6 mouse splenocytes stained with CD8 (clone 53-6.7) Alexa Fluor® 700 (filled histogram) or rat IgG2a, κ Alexa Fluor® 700 isotype control (open histogram).
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
100729 25 µg 68,00€
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100730 100 µg 156,00€
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, and immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
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  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
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  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
Product Citations
  1. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  2. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
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  12. Jackstadt R, et al. 2019. Cancer Cell. 36:319. PubMed
  13. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  14. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  15. Ying Zhang et al. 2017. Cancer cell. 32(3):377-391 . PubMed
  16. Kim MY, et al. 2017. Plant Biotechnol J. 1.729166667. PubMed
  17. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  18. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  19. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  20. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  21. Annu K, et al. 2020. Sci Rep. 10:2359. PubMed
  22. Qiao J, et al. 2019. Cancer Cell. 35:901. PubMed
  23. Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed
  24. Alexander RK, et al. 2020. eLife. 9:e54090.. PubMed
  25. Shimizu K, et al. 2020. Molecular Cell. 77(5):937-950.e6.. PubMed
  26. Resch TK, et al. 2018. Sci Rep. 0.722916667. PubMed
  27. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  28. Engelbertsen D, et al. 2013. Arterioscler Thromb Vasc Biol. 33:637. PubMed
  29. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  30. Bunn P, et al. 2014. J Immunol. 192:3709. PubMed
  31. Carty S, et al. 2014. PLoS One. 9:106659. PubMed
  32. Herz J, et al. 2015. J Exp Med. 212: 1153 - 1169. PubMed
  33. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  34. Solaymani-Mohammadi S, et al. 2016. J Leukoc Biol. 99: 475 - 482. PubMed
  35. Ma C, et al. 2016. Nature. 531:253-257. PubMed
  36. Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed
  37. Moodley D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9852 - 9857. PubMed
  38. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  39. Pérol L, et al. 2016. Nat Commun. 7:13027. PubMed
  40. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  41. Cerina M, et al. 2017. Brain Behav Immun. 59:103-117. PubMed
  42. Nakagawa P, et al. 2017. Physiol Rep. 10.14814/phy2.13084. PubMed
  43. Wang X, et al. 2020. Nat Commun. 4.505555556. PubMed
  44. Fang H, et al. 2020. Nat Commun. 4.661805556. PubMed
  45. Lewandowski JP, et al. 2020. Genome Biol. 1.039583333. PubMed
  46. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  47. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
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RRID
AB_493702 (BioLegend Cat. No. 100729)
AB_493703 (BioLegend Cat. No. 100730)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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