- Clone
- 53-6.7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T8, Lyt2, Ly-2
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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C57BL/6 mouse splenocytes were stained with CD3ε FITC and CD8a (clone 53-6.7) PerCP/Cyanine5.5, or Rat IgG2a, κ PerCP/Cyanine5.5 isotype control (bottom).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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100733 | 25 µg | 70€ | ||||
100734 | 100 µg | 190€ |
CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse thymus or spleen
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The CD8a antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is < 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. - Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
- Additional Product Notes
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BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
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Application References
(PubMed link indicates BioLegend citation) -
- Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
- Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
- Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
- Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
- Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
- Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
- Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
- Kamimura D, et al. 2006. J. Immunol. 177:306.
- Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
- Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
- Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
- Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
- Lee CH, et al. 2009. Clin. Cancer Res. PubMed
- Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
- Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
- Guo Y, et al. 2008. Blood 112:480. PubMed
- Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
- Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
- Kenna TJ, et al. 2008. Blood 111:2091. PubMed
- Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
- Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Medyouf H, et al. 2010. Blood 115:1175. PubMed
- Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
- Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
- Cui L, et al. 2015. J Control Release. 206:220. PubMed
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_2075239 (BioLegend Cat. No. 100733)
AB_2075238 (BioLegend Cat. No. 100734)
Antigen Details
- Structure
- Ig superfamily, CD8α chain, 34 kD
- Distribution
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Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
- Function
- Co-receptor for TCR
- Ligand/Receptor
- MHC class I molecule
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523. - Gene ID
- 12525 View all products for this Gene ID
- UniProt
- View information about CD8a on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
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PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse CD8a
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Biotin anti-mouse CD8a
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FITC anti-mouse CD8a
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PE anti-mouse CD8a
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PE/Cyanine5 anti-mouse CD8a
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Purified anti-mouse CD8a
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PE/Cyanine7 anti-mouse CD8a
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APC/Cyanine7 anti-mouse CD8a
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Alexa Fluor® 488 anti-mouse CD8a
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Alexa Fluor® 647 anti-mouse CD8a
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Pacific Blue™ anti-mouse CD8a
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Alexa Fluor® 700 anti-mouse CD8a
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PerCP/Cyanine5.5 anti-mouse CD8a
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PerCP anti-mouse CD8a
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Brilliant Violet 421™ anti-mouse CD8a
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Brilliant Violet 570™ anti-mouse CD8a
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Brilliant Violet 650™ anti-mouse CD8a
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Brilliant Violet 605™ anti-mouse CD8a
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Ultra-LEAF™ Purified anti-mouse CD8a
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Brilliant Violet 711™ anti-mouse CD8a
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Brilliant Violet 785™ anti-mouse CD8a
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Brilliant Violet 510™ anti-mouse CD8a
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Purified anti-mouse CD8a (Maxpar® Ready)
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Alexa Fluor® 594 anti-mouse CD8a
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PE/Dazzle™ 594 anti-mouse CD8a
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APC/Fire™ 750 anti-mouse CD8a
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GoInVivo™ Purified anti-mouse CD8a
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TotalSeq™-A0002 anti-mouse CD8a
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Spark Blue™ 550 anti-mouse CD8a
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Spark NIR™ 685 anti-mouse CD8a
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TotalSeq™-C0002 anti-mouse CD8a
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TotalSeq™-B0002 anti-mouse CD8a
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Spark YG™ 570 anti-mouse CD8a
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PE/Fire™ 640 anti-mouse CD8a
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PE/Fire™ 700 anti-mouse CD8a
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Spark Blue™ 574 anti-mouse CD8a Antibody
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Spark Violet™ 423 anti-mouse CD8a Antibody
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Spark UV™ 387 anti-mouse CD8a
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Spark Blue™ 515 anti-mouse CD8a
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