PerCP/Cyanine5.5 anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
H1dot2F3_PerCPCyanine55_CD69_Antibody_091919.png
PMA+ionomycin stimulated (6 hours) C57BL/6 mouse splenocytes stained with CD69 (clone H1.2F3) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram)
  • H1dot2F3_PerCPCyanine55_CD69_Antibody_091919.png
    PMA+ionomycin stimulated (6 hours) C57BL/6 mouse splenocytes stained with CD69 (clone H1.2F3) PerCP/Cyanine5.5 (filled histogram) or Armenian hamster IgG PerCP/Cyanine5.5 isotype control (open histogram)
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Avail. Save
104521 25 µg 80€
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104522 100 µg 220€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Additional Product Notes

View more applications data for this product in our Scientific Poster Library.

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  2. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  3. Tritama E, et al. 2018. Hum Vaccin Immunother. 14:1524. PubMed
  4. Andersen TK, et al. 2019. NPJ Vaccines. 4:9. PubMed
  5. Sinclair LV et al. 2019. Elife. 8 pii: e44210. PubMed
  6. Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed
  7. Tan L, et al. 2019. Cell Rep. 27:3657. PubMed
  8. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  9. Tomaru U et al. 2019. Cell reports. 26(3):639-651 . PubMed
  10. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  11. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  12. Ron–Harel N, et al. 2019. Cell Rep. 28:3011. PubMed
  13. Kim C, et al. 2019. Cell Rep. 29:2202. PubMed
  14. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  15. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  16. Nijnik A, et al. 2009. J Immunol. 183:5788. PubMed
  17. Lucca L, et al. 2014. J Immunol. 193:3267. PubMed
  18. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  19. Amor C, et al. 2020. Nature. 583:127. PubMed
  20. Lutes LK, et al. 2021. eLife. 10:00. PubMed
  21. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
RRID
AB_940497 (BioLegend Cat. No. 104521)
AB_2260065 (BioLegend Cat. No. 104522)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 4    Revision Date: 09.19.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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