APC anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
H1dot2F3_APC_083106
Con A-stimulated C57BL/6 mouse splenocytes (2 days) stained with H1.2F3 APC
  • H1dot2F3_APC_083106
    Con A-stimulated C57BL/6 mouse splenocytes (2 days) stained with H1.2F3 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
104513 25 µg 64€
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104514 100 µg 200€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Nenasheva T, et al. 2017. PLoS One. 12(6):e0178983. PubMed
  2. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  3. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  4. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  5. Yue X, et al. 2019. Nat Commun. 10:2011. PubMed
  6. Qi X, et al. 2019. Nat Commun. 10:2141. PubMed
  7. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  8. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  9. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  10. Mohammed RN, et al. 2019. Sci Rep. 4.185416667. PubMed
  11. Hojo MA, et al. 2019. Nat Commun. 2.224305556. PubMed
  12. Wang Z, et al. 2019. J Clin Invest. 130:4850. PubMed
  13. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  14. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  15. Xie X, et al. 2009. J Immunol. 182:7163. PubMed
  16. Xu L, et al. 2012. J Immunol. 188:248. PubMed
  17. Lo W, Allen D 2012. Nat Immunol. 13:880. PubMed
  18. Lin Y, et al. 2012. Cancer Prev Res. 5:1090. PubMed
  19. Stepanek O, et al. 2013. J Immunol. 190:1807. PubMed
  20. Michelet X, et al. 2015. J Immunol. 194:2079. PubMed
  21. Morrison V, et al. 2015. J Immunol. 195: 105 - 115. PubMed
  22. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
  23. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  24. Lebrun A, et al. 2015. J Immunol. 195: 4358 - 4368. PubMed
  25. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  26. Woyciechowski S, Hofmann M, Pircher H 2017. Eur J Immunol. 47:244-250. PubMed
  27. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  28. Zhuang Z, et al. 2021. J Exp Med. 218:00:00. PubMed
  29. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  30. Haque M, et al. 2021. STAR Protoc. 2:100264. PubMed
  31. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  32. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
RRID
AB_492844 (BioLegend Cat. No. 104513)
AB_492843 (BioLegend Cat. No. 104514)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.18.2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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