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PE/Cyanine7 anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
H1dot2F3_PECy7_091907
Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with H1.2F3 PE/Cyanine7
  • H1dot2F3_PECy7_091907
    Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with H1.2F3 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
104511 25 µg 62€
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104512 100 µg 152€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Oliver AJ, et al. 2020. Oncoimmunology. 9:1802979. PubMed
  2. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  3. Saha D et al. 2017. Cancer cell. 32(2):253-267 . PubMed
  4. Wilfahrt D, et al. 2021. Elife. 10:. PubMed
  5. Israelow B, et al. 2020. bioRxiv. . PubMed
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  8. Muris A, et al. 2012. J Immunol Methods. 381:59. PubMed
  9. Bally AP, et al. 2017. J Immunol. 198:205. PubMed
  10. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  11. Muthuswamy R, et al. 2021. J Immunother Cancer. 9:. PubMed
  12. Cannons JL, et al. 2021. Cell Rep. 37:109804. PubMed
  13. Manivasagam S, et al. 2022. J Immunol. 208:1341. PubMed
  14. Uhlig K, et al. 2015. J Virol. 89: 9044-9060. PubMed
  15. Christian LS, et al. 2021. Cell Reports. 35(6):109118. PubMed
  16. Wang D, et al. 2018. Immunity. 48:659. PubMed
  17. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  18. Israelow B, et al. 2021. Sci Immunol. 6:eabl4509. PubMed
  19. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  20. Xiong A, et al. 2022. EBioMedicine. 83:104239. PubMed
  21. Chung H, et al. 2021. Immune Netw. 21:e28. PubMed
  22. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  23. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  24. Silva M, et al. 2021. Sci Immunol. 6:eabf1152. PubMed
  25. Müller M, et al. 2021. Nat Commun. 12:7036. PubMed
  26. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  27. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
  28. Nelson SA, et al. 2022. NPJ Vaccines. 7:124. PubMed
  29. Levescot A, et al. 2021. J Clin Invest. 131:. PubMed
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  33. Imani J, et al. 2021. JCI Insight. 6:. PubMed
  34. Reed J, et al. 2020. Bio Protoc. 10:e3607. PubMed
  35. Nayak RR, et al. 2021. Cell Host Microbe. 29(3):362-377.e11. PubMed
  36. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  37. Becher J, et al. 2018. Dev Cell. 47:592. PubMed
  38. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  39. Toomer K, et al. 2016. J Immunol. 196: 3665 - 3676. PubMed
  40. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  41. Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed
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  44. Karandikar SH, et al. 2019. Anal Chem. 91:3405. PubMed
  45. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  46. Mendelsohn AC, et al. 2020. Am J Physiol Cell Physiol. 319:C997. PubMed
  47. Zhang C, et al. 2021. J Immunother Cancer. 9:. PubMed
  48. Dholakia J, et al. 2022. Gynecol Oncol. 164:170. PubMed
  49. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  50. Tsalavos S, et al. 2011. J Immunol. 186:6860. PubMed
  51. Liu Y, et al. 2020. Cell Death Dis. 11:1062. PubMed
  52. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  53. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  54. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  55. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  56. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  57. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
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  59. Ikeda T, et al. 2014. PLoS One. 9:115198. PubMed
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  61. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  62. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  63. Caetano MS, et al. 2019. Clin Cancer Res. 25:7576. PubMed
  64. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
  65. Pan Y, et al. 2021. NPJ Vaccines. 6:1. PubMed
  66. Chow AK, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. :. PubMed
  67. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
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  69. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
RRID
AB_493565 (BioLegend Cat. No. 104511)
AB_493564 (BioLegend Cat. No. 104512)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.18.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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