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FITC anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
h1.2f3
PMA-stimulated (6 hours) splenocytes stained with H1.2F3 FITC
  • h1.2f3
    PMA-stimulated (6 hours) splenocytes stained with H1.2F3 FITC
Compare all formats See FITC spectral data
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104505 50 µg 67€
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104506 500 µg 235€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Zheng X, et al. 2019. PLoS Pathog. 15:e1008036. PubMed
  2. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  3. Cordero-Reyes A, et al. 2016. J Am Heart Assoc. 5: 002484. PubMed
  4. Song Y, et al. 2020. Front Immunol. 11:558143. PubMed
  5. van Vloten JP, et al. 2022. J Immunother Cancer. 10:. PubMed
  6. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  7. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  8. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  9. Duan Q, et al. 2021. Front Cell Dev Biol. 9:761193. PubMed
  10. Burrack AL, et al. 2019. Cell Rep. 28:2140. PubMed
  11. Chen J et al. 2018. Cell reports. 25(12):3393-3404 . PubMed
  12. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  13. de Mingo Pulido , et al. 2021. Immunity. 54(6):1154-1167.e7. PubMed
  14. Byun JK, et al. 2020. Molecular Cell. 80(4):592-606.e8. PubMed
  15. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  16. Liu Q, et al. 2021. Cell Death Dis. 12:240. PubMed
  17. Demircioglu F, et al. 2020. Nat Commun. 11:1290. PubMed
  18. Bartleson JM, et al. 2020. Nat Immunol. 1384:21. PubMed
  19. Luff DH, et al. 2021. Front Immunol. 631271:12. PubMed
  20. Hasezaki T, et al. 2020. Sci Rep. 10:6969. PubMed
  21. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  22. Bhattacharjee P, et al. 2018. Sci Rep. 13:e0199785. PubMed
  23. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  24. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  25. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  27. Brummer G, et al. 2020. Oncogene. 39:2275. PubMed
  28. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  29. Mandrup OA, et al. 2021. Commun Biol. 310:4. PubMed
  30. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  31. Park D, et al. 2020. Cancer Res. 80:4172. PubMed
  32. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  33. Uhde A, et al. 2016. PLoS One. 11: 0161883. PubMed
  34. Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed
  35. Baier FA, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:745. PubMed
  36. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  37. Stanford S, et al. 2012. Proc Natl Acad Sci U S A. 109:13972. PubMed
  38. Haile S, et al. 2014. Cancer Immunol Res. 2:610. PubMed
  39. White C, et al. 2015. J Immunol. 194:697. PubMed
  40. Uddback I, et al. 2016. Sci Rep. 6:20137. PubMed
  41. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  42. Moore MJ et al. 2018. eLife. 7 pii: e33057. PubMed
  43. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
  44. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  45. XS R, et al. 2015. Diabetes. 64 90. PubMed
  46. Kang X, et al. 2022. J Immunol Res. 2022:8118577. PubMed
  47. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  48. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  49. Kretschmer L, et al. 2020. Nat Commun. 0.536805556. PubMed
  50. Tao F, et al. 2013. Biochem Pharmacol. 85:798. PubMed
  51. Ma K, et al. 2022. iScience. 25:104347. PubMed
  52. Riva A, et al. 2017. PLoS One.. 10.1371/journal.pone.0181964. PubMed
  53. Martina M, et al. 2016. J Am Soc Nephrol. 27: 1113-1123. PubMed
  54. Wang L, et al. 2021. Sci Adv. 7:eabj4796. PubMed
  55. Jing Y, et al. 2021. Front Immunol. 12:651860. PubMed
  56. Niven J, et al. 2019. Cell Rep. 28:21. PubMed
  57. Kiss M, et al. 2020. Cancer Immunol Res. 9:309. PubMed
  58. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  59. Tsyklauri O, et al. 2021. EMBO Rep. 22:e50785. PubMed
  60. Rui J, et al. 2021. Nat Commun. 12:5074. PubMed
  61. Song TY, et al. 2021. Nat Commun. 12:7003. PubMed
  62. Klezovich-Bénard M, et al. 2012. PLoS One. 8:e1002481. PubMed
  63. Skirecki T, et al. 2020. JCI Insight. 5:. PubMed
  64. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  65. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  66. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  67. Kuhn NF et al. 2019. Cancer cell. 35(3):473-488 . PubMed
  68. Han J, et al. 2015. Cancer Res . 75: 5273 - 5282. PubMed
  69. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID
AB_313108 (BioLegend Cat. No. 104505)
AB_313109 (BioLegend Cat. No. 104506)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.18.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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