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Purified anti-mouse CD69 Antibody

Pricing & Availability
Clone
H1.2F3 (See other available formats)
Regulatory Status
RUO
Other Names
Very Early Activation Antigen (VEA), AIM, EA1, MLR3, gp34/28
Isotype
Armenian Hamster IgG
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Product Citations
publications
H1dot2F3_Pure_091907
Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with purified H1.2F3, followed by anti-Armenian hamster IgG FITC
  • H1dot2F3_Pure_091907
    Con A-stimulated Balb/c mouse splenocytes (24 hours) stained with purified H1.2F3, followed by anti-Armenian hamster IgG FITC
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104502 500 µg 203€
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Description

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3, and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse dendritic epidermal T cell line Y245
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The H1.2F3 antibody has been reported to augment T cell activation. Additional reported applications (for the relevant formats) include: in vitro T cell and NK cell activation1-3, immunohistochemistry4,5, and immunoprecipitation1.

This antibody has been characterized in the literature as containing a lambda (?) light chain.

Application References

(PubMed link indicates BioLegend citation)
  1. Yokoyama WM, et al. 1988. J. Immunol. 141:369. (IP)
  2. Sobel ES, et al. 1993. J. Immunol. 150:673.
  3. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  4. Zhou X, et al. 2005. J. Biol. Chem. 280:31240. (IHC)
  5. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
  6. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  7. Lee JW, et al. 2006. Nature Immunol. 8:181.
  8. Epardaud M, et al. 2008. Cancer Res. 15:2972. PubMed
  9. Jordan JM, et al. 2008. 76:3717. PubMed
  10. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  11. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
Product Citations
  1. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  2. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  3. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  4. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  5. Guo R, et al. 2020. Cell Res. 30:21. PubMed
  6. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  7. Xu L, et al. 2017. Int J Biol Macromol. . 10.1016/j.ijbiomac.2017.07.090. PubMed
  8. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  9. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  10. Carpenter SM, et al. 2017. PLoS Pathog. 13:e1006704. PubMed
  11. Oda SK, et al. 2020. J Exp Med. 217: . PubMed
  12. Niss Arfelt K, et al. 2017. Blood. 129:866. PubMed
  13. Kroczek AL, et al. 2019. Front Immunol. 9:2806. PubMed
  14. Morabito KM, et al. 2017. Mucosal Immunol. 0.795138889. PubMed
  15. Pawaria S, et al. 2020. Arthritis Rheumatol. 72:359. PubMed
  16. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  17. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  18. Kiripolsky J, et al. 2021. J Autoimmun. 118:102608. PubMed
  19. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  20. Snell LM, et al. 2018. Immunity. 49:678. PubMed
  21. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  22. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  23. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  24. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  25. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  26. Hsiao CC, et al. 2021. Cells. 10:. PubMed
  27. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  28. Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
  29. Schuler CF, et al. 2020. Allergy. 75:2279. PubMed
  30. Ishikawa C, et al. 2013. Biochim Biophys Acta. 167:99. PubMed
  31. Park GY, et al. 2020. Immune Netw. e43:20. PubMed
  32. Kenna T, et al. 2008. Blood. 111:2091. PubMed
  33. Clark K, et al. 2019. Front Immunol. 10:2355. PubMed
  34. Karmaus P, et al. 2011. J Leukoc Biol. 90:983. PubMed
  35. Roy S, et al. 2018. Cancer Res. 78:5600. PubMed
  36. Brownlie D, et al. 2021. J Immunother Cancer. 9:. PubMed
  37. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  38. Wagner AK, et al. 2017. Nat Commun. 8:15627. PubMed
  39. Cui X, et al. 2017. J Immunol. 199:4066. PubMed
  40. Scutts SR, et al. 2018. Cell Rep. 25:1953. PubMed
  41. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  42. Cané S, et al. 2021. J Immunother Cancer. 9:. PubMed
  43. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  44. Richards KA, et al. 2018. Front Immunol. 0.829861111. PubMed
  45. Englezou PC, et al. 2018. Mol Ther Nucleic Acids. 12:118. PubMed
  46. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  47. Rios–Doria J, et al. 2017. Cancer Res. 77:2686. PubMed
  48. Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
  49. Epardaud M, et al. 2008. Cancer Res. 68:2972. PubMed
  50. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  51. Sutiwisesak R, et al. 2020. PLoS Pathog. 16:e1009000. PubMed
RRID
AB_313105 (BioLegend Cat. No. 104502)

Antigen Details

Structure
C-type lectin, 27/33 kD
Distribution

Activated T cells and B cells, NK cells, granulocytes, thymocytes, platelets

Function
Lymphocyte activation
Cell Type
B cells, Granulocytes, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Testi R, et al. 1994. Immunol. Today 15:479.
3. Moretta A, et al. 1991. J. Exp. Med. 174:1393.
4. Yokoyama WM, et al. 1988. J. Immunol. 141:369.

Gene ID
12515 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 04.18.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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