Loading...

FITC anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
m5/114.15.2 FITC
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
  • m5/114.15.2 FITC
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
107605 50 µg 57€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
107606 500 µg 176€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  2. Nguyen T, et al. 2021. STAR Protoc. 2:101004. PubMed
  3. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  4. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  5. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  6. Sim SL, et al. 2022. Front Endocrinol (Lausanne). 13:957833. PubMed
  7. Tarban N, et al. 2022. Cells. 11:. PubMed
  8. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  9. Ma J, et al. 2020. Adv Sci (Weinh). 7:2000609. PubMed
  10. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  11. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  12. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  13. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  14. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  15. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  16. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  17. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  18. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  19. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  20. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  21. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  22. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  23. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  24. Matsumura F, et al. 2021. J Immunol. 207:938. PubMed
  25. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  26. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  27. Renga G, et al. 2020. Life Sci Alliance. 3:. PubMed
  28. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  29. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  30. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  31. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  32. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  33. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  34. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  35. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  36. Mainz RE, et al. 2022. Cells. 11:. PubMed
  37. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  38. Carozza JA, et al. 2020. Nat Cancer. 184:1. PubMed
  39. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  40. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  41. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  42. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  43. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  44. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  45. Kang SJ, et al. 2022. Food Sci Anim Resour. 42:903. PubMed
  46. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  47. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
  48. Alikhanyan K, et al. 2021. Cancers (Basel). 13:. PubMed
  49. Zou J, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:983. PubMed
  50. Dobosz E, et al. 2021. Dis Model Mech. :14. PubMed
  51. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  52. Koma T, et al. 2014. J Virol. 88:7178. PubMed
  53. Pradhan K, et al. 2021. Front Immunol. 12:778830. PubMed
  54. White C, et al. 2015. J Immunol. 194:697. PubMed
  55. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  56. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
  57. Donaldson B, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0270-1. PubMed
  58. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  59. Hakim R, et al. 2019. Stem Cell Res Ther. 10:115. PubMed
  60. Kramer K, et al. 2017. ACS Omega. 0.240972222. PubMed
  61. Vorselen D, et al. 2021. Elife. 10:. PubMed
  62. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  63. Dewi IMW, et al. 2021. Cell Reports Medicine. 2(5):100289. PubMed
  64. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  65. Steinbach K, et al. 2013. J Immunol. 191:4531. PubMed
  66. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  67. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  68. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  69. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  70. Guo S, et al. 2021. Sci Rep. 11:23745. PubMed
  71. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  72. Zhao L, et al. 2021. STAR Protocols. 2(3):100732. PubMed
  73. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  74. Banovic T, et al. 2009. J Immunol. 182:912. PubMed
  75. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  76. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  77. Hidaka T, et al. 2016. Nat Immunol. 18:64-73. PubMed
  78. Budai Z, et al. 2021. Cells. 10:. PubMed
  79. Luo M, et al. 2021. J Immunol. 207:1683. PubMed
  80. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  81. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  82. Wu J, et al. 2019. J Immunol Res. 2019:1749803. PubMed
  83. Kelly SH, et al. 2021. Adv Healthc Mater. 10:e2001614. PubMed
  84. Schulz A, et al. 2015. J Biol Chem. 211: 553 - 567. PubMed
  85. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  86. Wijayadikusumah A, et al. 2017. Amino Acids. 10.1007/s00726-017-2466-6. PubMed
  87. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  88. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  89. Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed
  90. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
  91. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  92. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  93. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  94. Xiao Z, et al. 2022. Mater Today Bio. 15:100297. PubMed
  95. Nicholls J, et al. 2021. Front Cell Dev Biol. 9:737880. PubMed
  96. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  97. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  98. Harberts E, et al. 2015. J Immunol. 194:3127. PubMed
  99. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  100. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  101. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  102. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  103. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  104. Al-Zaeed N, et al. 2021. Cell Death Dis. 12:611. PubMed
  105. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  106. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  107. Dong S, et al. 2007. Int Immunopharmacol. 7:725. PubMed
  108. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
  109. Deets KA, et al. 2021. Elife. 10:. PubMed
RRID
AB_313320 (BioLegend Cat. No. 107605)
AB_313321 (BioLegend Cat. No. 107606)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 4    Revision Date: 10.30.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse I-A/I-E

  • FITC anti-mouse I-A/I-E

  • PE anti-mouse I-A/I-E

  • Purified anti-mouse I-A/I-E

  • PE/Cyanine5 anti-mouse I-A/I-E

  • APC anti-mouse I-A/I-E

  • Alexa Fluor® 488 anti-mouse I-A/I-E

  • Alexa Fluor® 647 anti-mouse I-A/I-E

  • Pacific Blue™ anti-mouse I-A/I-E

  • Alexa Fluor® 700 anti-mouse I-A/I-E

  • PerCP/Cyanine5.5 anti-mouse I-A/I-E

  • PerCP anti-mouse I-A/I-E

  • APC/Cyanine7 anti-mouse I-A/I-E

  • PE/Cyanine7 anti-mouse I-A/I-E

  • Brilliant Violet 421™ anti-mouse I-A/I-E

  • Brilliant Violet 510™ anti-mouse I-A/I-E

  • Purified anti-mouse I-A/I-E (Maxpar® Ready)

  • Brilliant Violet 605™ anti-mouse I-A/I-E

  • Brilliant Violet 650™ anti-mouse I-A/I-E

  • Brilliant Violet 711™ anti-mouse I-A/I-E

  • Brilliant Violet 785™ anti-mouse I-A/I-E

  • PE/Dazzle™ 594 anti-mouse I-A/I-E

  • Alexa Fluor® 594 anti-mouse I-A/I-E

  • APC/Fire™ 750 anti-mouse I-A/I-E

  • TotalSeq™-A0117 anti-mouse I-A/I-E

  • Ultra-LEAF™ Purified anti-mouse I-A/I-E

  • TotalSeq™-B0117 anti-mouse I-A/I-E

  • TotalSeq™-C0117 anti-mouse I-A/I-E

  • Spark Blue™ 550 anti-mouse I-A/I-E

  • PE/Fire™ 640 anti-mouse I-A/I-E

  • Spark YG™ 581 anti-mouse I-A/I-E

  • PE/Fire™ 810 anti-mouse I-A/I-E

  • Spark UV™ 387 anti-mouse I-A/I-E
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account