Loading...

APC/Cyanine7 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, ? APC/Cyanine7 isotype control (right).
  • M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
107627 25 µg 76€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
107628 100 µg 234€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, in vitro and in vivo blocking of antigen presentation or ligand binding4-7, and spatial biology (IBEX)17,18. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
  17. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  18. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Garber C, et al. 2019. Nat Neurosci. 1.802777778. PubMed
  2. Wong Fok Lung T, et al. 2020. Nat Microbiol. 141:5. PubMed
  3. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  4. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  5. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  6. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  7. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  8. Li Y, et al. 2021. Basic Res Cardiol. 116:30. PubMed
  9. Chen WS, et al. 2021. Cell Rep. 37:109974. PubMed
  10. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  11. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  12. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  13. Li K, et al. 2022. J Immunother Cancer. 10:. PubMed
  14. Super M, et al. 2021. Nat Biomed Eng. Online ahead of print. PubMed
  15. Zhao Y, et al. 2021. Int Immunopharmacol. 93:107406. PubMed
  16. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  17. Liang Z, et al. 2020. Pain. 161:2089. PubMed
  18. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  19. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  20. Peres-Emidio EC, et al. 2022. Front Cell Infect Microbiol. 12:811474. PubMed
  21. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  22. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  23. Bellelli R et al. 2018. Molecular cell. 70(4):707-721 . PubMed
  24. Riquelme SA, et al. 2020. Cell Metabolism. 31(6):1091-1106.e6. PubMed
  25. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  26. DeVito NC, et al. 2021. Cell Reports. 35(5):109071. PubMed
  27. Jia Q, et al. 2021. Theranostics. 4699:11. PubMed
  28. Xu Z, et al. 2020. Cancer Immunol Res. 1354:8. PubMed
  29. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  30. Acker KP, et al. 2019. iScience. 19:281. PubMed
  31. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  32. Bauer T, et al. 2021. Life (Basel). 11:. PubMed
  33. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  34. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  35. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  36. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  37. França TT, et al. 2021. JCI Insight. 6:. PubMed
  38. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  39. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  40. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  41. Choi EW, et al. 2020. Sci Rep. 10:12001. PubMed
  42. Liu C et al. 2019. Immunity. 51(2):381-397 . PubMed
  43. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  44. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  45. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  46. Bhattacharya A, et al. 2018. Neuropsychopharmacology. 43:2586. PubMed
  47. Yang B, et al. 2021. Cell Biosci. 11:28. PubMed
  48. Han Y, et al. 2022. Int J Biol Sci. 18:5653. PubMed
  49. Lowe PP, et al. 2020. J Neuroinflammation. 0.913888889. PubMed
  50. Bent EH, et al. 2021. Nat Commun. 12:6218. PubMed
  51. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  52. Hofmann J, et al. 2021. Front Immunol. 11:599495. PubMed
  53. Mohammadi MR, et al. 2021. Commun Biol. 4:685. PubMed
  54. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  55. Maniati E, et al. 2020. Cell Rep. 30:525. PubMed
  56. Sung PS, et al. 2022. JCI Insight. :. PubMed
  57. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  58. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  59. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  60. Li J, et al. 2019. iScience. 16:468. PubMed
  61. Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
  62. Willemsen J, et al. 2021. Cell Rep. 37:109977. PubMed
  63. Ding L et al. 2018. Cell reports. 25(11):2972-2980 . PubMed
  64. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
  65. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  66. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  67. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  68. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  69. Xu C, et al. 2021. Cell Reports. 35(11):109235. PubMed
  70. Moreno M et al. 2014. The Journal of Neuroscience. 34(24):8175-85 . PubMed
  71. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  72. Lavoie S, et al. 2020. Gastroenterology. 158:1359. PubMed
  73. Gundra UM, et al. 2017. Nat Immunol. 1.195833333. PubMed
  74. Seenappa LM, et al. 2022. NPJ Vaccines. 7:128. PubMed
  75. Rosen SF, et al. 2022. Genome Med. 14:108. PubMed
  76. Sheng D, et al. 2022. J Immunother Cancer. 10:. PubMed
  77. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  78. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  79. Smith HJ, et al. 2018. Cancer. 124:4657. PubMed
  80. Boguslawski KM, et al. 2020. Sci Adv. 6:eaax7515. PubMed
  81. Wang L, et al. 2020. J Nanobiotechnology. 18:38. PubMed
  82. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  83. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  84. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  85. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  86. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  87. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  88. Deniset JF et al. 2019. Immunity. 51(1):131-140 . PubMed
  89. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
  90. Deets KA, et al. 2021. Elife. 10:. PubMed
  91. Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
RRID
AB_1659252 (BioLegend Cat. No. 107627)
AB_2069377 (BioLegend Cat. No. 107628)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All I-A/I-E Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse I-A/I-E M5/114.15.2 FC
FITC anti-mouse I-A/I-E M5/114.15.2 FC
PE anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
PE/Cyanine5 anti-mouse I-A/I-E M5/114.15.2 FC
APC anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 488 anti-mouse I-A/I-E M5/114.15.2 FC,3D IHC
Alexa Fluor® 647 anti-mouse I-A/I-E M5/114.15.2 FC
Pacific Blue™ anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 700 anti-mouse I-A/I-E M5/114.15.2 FC,SB
PerCP/Cyanine5.5 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP anti-mouse I-A/I-E M5/114.15.2 FC
APC/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Cyanine7 anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 421™ anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,SB
Brilliant Violet 510™ anti-mouse I-A/I-E M5/114.15.2 FC
Purified anti-mouse I-A/I-E (Maxpar® Ready) M5/114.15.2 FC,CyTOF®
Brilliant Violet 605™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 650™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 711™ anti-mouse I-A/I-E M5/114.15.2 FC
Brilliant Violet 785™ anti-mouse I-A/I-E M5/114.15.2 FC
PE/Dazzle™ 594 anti-mouse I-A/I-E M5/114.15.2 FC
Alexa Fluor® 594 anti-mouse I-A/I-E M5/114.15.2 IHC-F,3D IHC
APC/Fire™ 750 anti-mouse I-A/I-E M5/114.15.2 FC
TotalSeq™-A0117 anti-mouse I-A/I-E M5/114.15.2 PG
Ultra-LEAF™ Purified anti-mouse I-A/I-E M5/114.15.2 FC,IHC-F,IP,Block
TotalSeq™-B0117 anti-mouse I-A/I-E M5/114.15.2 PG
TotalSeq™-C0117 anti-mouse I-A/I-E M5/114.15.2 PG
Spark Blue™ 550 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 640 anti-mouse I-A/I-E M5/114.15.2 FC
Spark YG™ 581 anti-mouse I-A/I-E M5/114.15.2 FC
PE/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Spark UV™ 387 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Violet™ 538 anti-mouse I-A/I-E M5/114.15.2 FC
PerCP/Fire™ 806 anti-mouse I-A/I-E M5/114.15.2 FC
Spark Red™ 718 anti-mouse I-A/I-E M5/114.15.2 FC
APC/Fire™ 810 anti-mouse I-A/I-E M5/114.15.2 FC
Go To Top Version: 5    Revision Date: 02.01.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account