Alexa Fluor® 647 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_A647_Ly6G_Antibody_1_FC_051816
C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647
  • 1A8_A647_Ly6G_Antibody_1_FC_051816
    C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647
  • 1A8_A647_Ly6G_Antibody_2_IHCF_051816
    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat/mouse serum for 30 minutes at room temperature. Then the section was stained with 5 µg/mL anti-mouse Ly-6G (clone 1A8) Alexa Fluor® 647 (red), anti-mouse/human CD45R/B220 (clone RA3-6B2) Brilliant Violet 510™ (green) and CD8a (clone 53-6.7) Brilliant Violet 421™ (blue) overnight at 4°C. The image was captured with a 10X objective.
Compare all formats See Alexa Fluor® 647 spectral data See high resolution IHC data...
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127609 25 µg 100€
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127610 100 µg 212€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
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  5. Miyatake Y, et al. 2018. J Immunol. 201:3051. PubMed
  6. Prates D, et al. 2011. J Leukoc Biol. 90:575. PubMed
  7. Orberg E, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.53. PubMed
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  9. Barkaway A, et al. 2021. Immunity. . PubMed
  10. Johnson JL, et al. 2022. Nat Commun. 13:5529. PubMed
  11. Golden GJ, et al. 2021. MBio. 12:e0118121. PubMed
  12. Lechuga-Vieco AV, et al. 2020. Sci Adv. 6:eaba5345. PubMed
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  15. Chang T, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006485. PubMed
  16. Kolawole A, et al. 2015. J Virol. 90: 1499-1506. PubMed
  17. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
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  19. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  20. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  21. Kretschmer S, et al. 2016. Lab Invest. 10.1038/labinvest.2016.69. PubMed
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  24. Wang H, et al. 2022. Curr Protoc. 2:e446. PubMed
  25. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  26. Saithong S, et al. 2021. J Inflamm Res. 14:2333. PubMed
  27. Ueki H, et al. 2020. Nat Protoc. 15:1041. PubMed
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  33. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  34. Ghaffari S, et al. 2020. Arterioscler Thromb Vasc Biol. ATVBAHA120314557:. PubMed
  35. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  36. Ganguly K, et al. 2022. Front Immunol. 13:930449. PubMed
  37. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  38. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  39. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  40. Myerson JW, et al. 2021. Nat Nanotechnol. Online ahead of print. PubMed
  41. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  42. Jyh Liang Hor, William R. Heath, Scott N. Mueller 2017. Sci Rep. 7:41091. PubMed
  43. Chung L et al. 2018. Cell host & microbe. 23(2):203-214 . PubMed
  44. Oswald DM, et al. 2020. Glycoconj J. 37:395. PubMed
  45. NaveenKumar SK, et al. 2020. J Pineal Res. 69:e12676. PubMed
  46. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  47. Koikawa K, et al. 2021. Cell. 184(18):4753-4771.e27. PubMed
  48. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  49. Niemi JP, et al. 2020. Methods Mol Biol. 2143:207. PubMed
  50. Ge C, et al. 2020. Cell Reports. 29(13):4236-4244.e3.. PubMed
  51. Li J, et al. 2014. Infect Immun . 82:1579. PubMed
  52. Martinod K, et al. 2015. Blood. 125:1948. PubMed
  53. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  54. Shahid M, et al. 2020. Sci Rep. 0.901388889. PubMed
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RRID
AB_1134162 (BioLegend Cat. No. 127609)
AB_1134159 (BioLegend Cat. No. 127610)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F
Purified anti-mouse Ly-6G 1A8 FC, IHC-F, IHC
Biotin anti-mouse Ly-6G 1A8 FC, IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC, IHC-F
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC, IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC, IHC-F, SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC, IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC, Depletion, IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC, CyTOF®, WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Go To Top Version: 5    Revision Date: 01.06.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Alexa Fluor® 594 anti-mouse Ly-6G

  • Purified anti-mouse Ly-6G

  • Biotin anti-mouse Ly-6G

  • FITC anti-mouse Ly-6G

  • PE anti-mouse Ly-6G

  • Alexa Fluor® 647 anti-mouse Ly-6G

  • Pacific Blue™ anti-mouse Ly-6G

  • APC anti-mouse Ly-6G

  • PerCP/Cyanine5.5 anti-mouse Ly-6G

  • PE/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 700 anti-mouse Ly-6G

  • APC/Cyanine7 anti-mouse Ly-6G

  • Alexa Fluor® 488 anti-mouse Ly-6G

  • Brilliant Violet 421™ anti-mouse Ly-6G

  • Brilliant Violet 570™ anti-mouse Ly-6G

  • Ultra-LEAF™ Purified anti-mouse Ly-6G

  • Brilliant Violet 510™ anti-mouse Ly-6G

  • Purified anti-mouse Ly-6G (Maxpar® Ready)

  • Brilliant Violet 650™ anti-mouse Ly-6G

  • Brilliant Violet 711™ anti-mouse Ly-6G

  • Brilliant Violet 605™ anti-mouse Ly-6G

  • Brilliant Violet 785™ anti-mouse Ly-6G

  • PE/Dazzle™ 594 anti-mouse Ly-6G

  • APC/Fire™ 750 anti-mouse Ly-6G

  • PerCP anti-mouse Ly-6G

  • TotalSeq™-A0015 anti-mouse Ly-6G

  • TotalSeq™-C0015 anti-mouse Ly-6G

  • TotalSeq™-B0015 anti-mouse Ly-6G

  • Spark Blue™ 550 anti-mouse Ly-6G

  • Spark NIR™ 685 anti-mouse Ly-6G

  • Spark YG™ 593 anti-mouse Ly-6G

  • APC/Fire™ 810 anti-mouse Ly-6G Antibody

  • PE/Cyanine5 anti-mouse Ly-6G

  • PE/Fire™ 810 anti-mouse Ly-6G Antibody

  • Spark UV™ 387 anti-mouse Ly-6G

  • PE/Fire™ 640 anti-mouse Ly-6G

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