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Alexa Fluor® 488 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_AF488_071510
C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1.4 Alexa Fluor® 488
  • HK1dot4_AF488_071510
    C57BL/6 bone marrow cells stained with anti-mouse Ly-6C, HK1.4 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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128021 25 µg 100€
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128022 100 µg 212€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Baratchart E, et al. 2022. PLoS Comput Biol. 18:e1009839. PubMed
  2. Liu J, et al. 2019. Immunity. 50:600. PubMed
  3. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  4. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  5. Alexander J, et al. 2012. Kidney Int. 82:961. PubMed
  6. Vaibhav K, et al. 2018. J Exp Med. 215:2636. PubMed
  7. Lu X, et al. 2020. Sci Transl Med. 12:. PubMed
  8. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  9. Zenker S, et al. 2014. J Immunol. 192:2830. PubMed
  10. Zhou Z, et al. 2022. Life (Basel). 12:. PubMed
  11. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  12. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  13. Dang MT, et al. 2021. Cell Reports. 34(13):108917. PubMed
  14. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  15. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  16. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  17. Toomey C, et al. 2015. Proc Natl Acad Sci U S A. 112:3040. PubMed
  18. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  19. Liu Z, et al. 2020. Cell. 178(6):1509-1525.e19.. PubMed
  20. Geng T, et al. 2021. J Infect Dis. 223:2186. PubMed
  21. Stephens WZ, et al. 2021. Cell Rep. 37:109916. PubMed
  22. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  23. Lo CH, et al. 2021. Sci Rep. 6055:11. PubMed
  24. Breznik JA, et al. 2021. The Journal of Physiology. 599(8):2169-2195. PubMed
  25. Breznik JA, et al. 2021. Immunohorizons. 5:370. PubMed
  26. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  27. Skokowa J, et al. 2022. Nat Commun. 13:2948. PubMed
  28. Galuppo P, et al. 2017. FASEB J. . 10.1096/fj.201700317R. PubMed
  29. Kelley WJ, et al. 2022. JCI Insight. 7: . PubMed
  30. Maier N, et al. 2014. J Immunol. 192:763. PubMed
  31. Fujimura N, et al. 2016. Sci Rep. 5:11664. PubMed
  32. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  33. Bozoglu T, et al. 2022. Adv Sci (Weinh). 9:e2103867. PubMed
  34. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
  35. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  36. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  37. Ishihara J, et al. 2018. Nat Commun. 9:2163. PubMed
  38. Ehrens A, et al. 2021. Cell Reports. 34(2):108621. PubMed
  39. Liu Z, et al. 2020. STAR Protoc. 1:100029. PubMed
  40. Song W, et al. 2022. Immunity. 55:290. PubMed
  41. Yu YR, et al. 2020. STAR Protocols. 11(3):e0150606. PubMed
  42. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
RRID
AB_10640820 (BioLegend Cat. No. 128021)
AB_10639728 (BioLegend Cat. No. 128022)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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