Alexa Fluor® 700 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
publications
1A8_AF700_072210
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
  • 1A8_AF700_072210
    C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
Compare all formats See Alexa Fluor® 700 spectral data
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127621 25 µg 93€
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127622 100 µg 199€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Schädlich IS, et al. 2022. iScience. 25:104470. PubMed
  2. Bruggemann TR, et al. 2022. iScience. 25:105185. PubMed
  3. Gallizioli M, et al. 2020. Cell Rep. 33:108291. PubMed
  4. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  5. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  6. Dong L, et al. 2021. Cancer Cell. . PubMed
  7. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  8. Haertel E, et al. 2018. Eur J Immunol. 48:1001. PubMed
  9. Esmaili S, et al. 2021. Cell Systems. 12(5):432-445.e7. PubMed
  10. Salas–Pérdomo A, et al. 2019. Sci Rep. 9:8309. PubMed
  11. Ma C, et al. 2018. Science. 360:eaan5931. PubMed
  12. Liang P, et al. 2022. Mol Biomed. 3:14. PubMed
  13. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  14. Tuit S, et al. 2019. Cell Rep. 29:1221. PubMed
  15. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  16. Mainz RE, et al. 2022. Cells. 11:. PubMed
  17. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  18. Stewart JM, et al. 2020. ACS Biomater Sci Eng. 6:5941. PubMed
  19. Lin J, et al. 2017. Sci Rep. 7:41722. PubMed
  20. Hartwig T et al. 2018. Cell reports. 25(13):3564-3572 . PubMed
  21. Lu CY, et al. 2020. J Neurosci. 40:9602. PubMed
  22. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  23. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
  24. Kren N, et al. 2020. Sci Rep. 10:7390. PubMed
  25. Lewis EL, et al. 2021. Front Immunol. 12:741518. PubMed
  26. Ahadome S, et al. 2016. JCI Insight. 1:e87012. PubMed
  27. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  28. Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
  29. Gilbert NM, et al. 2021. Biol Open. 10:. PubMed
  30. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  31. Toomey CB, et al. 2018. Invest Ophthalmol Vis Sci. 59:662. PubMed
  32. Da Silva CG, et al. 2019. Theranostics. 4.878472222. PubMed
  33. Henrich IC, et al. 2021. Cancer Res. 81:2171. PubMed
  34. Toomey C, et al. 2015. Proc Natl Acad Sci U S A. 112:3040. PubMed
  35. Jin C, et al. 2019. Cell. 176:998. PubMed
  36. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  37. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  38. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  39. Godwin MS, et al. 2019. JCI Insight. 4:e126070. PubMed
  40. Otxoa–de–Amezaga A, et al. 2019. Stroke. 50:1548. PubMed
  41. Youshani AS, et al. 2019. J Neuroinflammation. 16:25. PubMed
  42. Soberanes S et al. 2018. Cell metabolism. 29(2):335-347 . PubMed
  43. Nalbandian A, et al. 2017. Inflammation. 40:21:00. PubMed
  44. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  45. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  46. Molgora M, et al. 2020. Cell. 182:886. PubMed
  47. Grube J, et al. 2022. Cell Death Dis. 13:704. PubMed
  48. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  49. Celorrio M, et al. 2022. Acta Neuropathol Commun. 10:10. PubMed
  50. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  51. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
  52. Smith R, et al. 2016. J Leukoc Biol. 100:371-80. PubMed
  53. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  54. Chen D, et al. 2021. JCI Insight. 6:. PubMed
  55. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  56. Flak MB, et al. 2020. J Clin Invest. 130:359. PubMed
  57. Fearon AE, et al. 2021. iScience. 24:103143. PubMed
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  61. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
RRID
AB_10640452 (BioLegend Cat. No. 127621)
AB_10643269 (BioLegend Cat. No. 127622)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Go To Top Version: 4    Revision Date: 01.06.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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