APC/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
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Product Citations
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1A8_APCCy7_072210
C57BL/6 bone marrow cells stained with 1A8 APC/Cyanine7
  • 1A8_APCCy7_072210
    C57BL/6 bone marrow cells stained with 1A8 APC/Cyanine7
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
127623 25 µg 72€
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127624 100 µg 220€
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  2. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  3. Van Winkle JA et al. 2018. Cell host & microbe. 24(5):665-676 . PubMed
  4. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  5. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  6. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  7. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  8. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  9. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  10. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  11. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
  12. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  13. Ho YH, et al. 2019. Cell Stem Cell. 25:407. PubMed
  14. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  15. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  16. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  17. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  18. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  19. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
  20. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  21. Murphy M, et al. 2017. Eur J Immunol. 47:880. PubMed
  22. Dodd RD, et al. 2017. Cancer Res. 77:4486. PubMed
  23. Anderson MC, et al. 2018. Cell Chem Biol. 25:483. PubMed
  24. Namkoong H, et al. 2018. PLoS Pathog. 14:e1006955. PubMed
  25. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  26. Delás MJ, et al. 2019. Cell Rep. 27:719. PubMed
  27. Yousef H, et al. 2019. Nat Med. 25:988. PubMed
  28. Burgener SS, et al. 2019. Cell Rep. 27:3646. PubMed
  29. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  30. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  31. Kovács B, et al. 2020. Front Immunol. 2.508333333. PubMed
  32. Qi S, et al. 2020. Theranostics. 10:1814. PubMed
  33. Yu X, et al. 2020. Nat Commun. 11:1110. PubMed
  34. Currier MA, et al. 2017. Oncotarget. 8:17412. PubMed
  35. Lee MC, et al. 2017. J Immunol. 198:3565. PubMed
  36. Buzzelli JN, et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316:G251. PubMed
  37. Quatrini L, et al. 2018. Nat Immunol. 19:954. PubMed
  38. Saleh R, et al. 2018. J Immunol. 201:2042. PubMed
  39. Briukhovetska D, et al. 2020. Frontiers in Immunology. 1.428472222. PubMed
  40. Cai W, et al. 2019. J Neuroinflammation. 0.788194444. PubMed
  41. Kalafati L, et al. 2020. Cell. 183:771. PubMed
  42. Price P, et al. 2014. J Virol. 88:10840. PubMed
  43. Hyatt L, et al. 2014. PLoS One. 9:109072. PubMed
  44. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  45. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  46. Fontana MF, et al. 2015. Parasite Immunol. 37: 470-478. PubMed
  47. Hilliard K, et al. 2015. Infect Immun . 83: 4015 - 4027. PubMed
  48. Zhang J, et al. 2015. Sci Rep. 5: 15553. PubMed
  49. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  50. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  51. Woods T, et al. 2016. J Virol. 90: 2783 - 2793. PubMed
  52. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  53. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
  54. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  55. Wilson R, et al. 2016. Nat Commun. 7:13597. PubMed
RRID
AB_10645331 (BioLegend Cat. No. 127623)
AB_10640819 (BioLegend Cat. No. 127624)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01.06.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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