FITC anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
m5/114.15.2 FITC
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
  • m5/114.15.2 FITC
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) FITC (solid line) or rat IgG2b, κ FITC isotype control (dashed line).
See FITC spectral data
Cat # Size Price Quantity Avail. Save
107605 50 µg 48€
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107606 500 µg 160€
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Wijayadikusumah A, et al. 2017. Amino Acids. 10.1007/s00726-017-2466-6. PubMed
  2. Donaldson B, et al. 2017. J Immunother Cancer. 10.1186/s40425-017-0270-1. PubMed
  3. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  4. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  5. Hernández–Santos N, et al. 2018. Cell Host Microbe. 1.313194444. PubMed
  6. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  7. Zwick M, et al. 2019. Front Immunol. 10:222. PubMed
  8. Sultan H, et al. 2018. Cancer Immunol Res. 6:617. PubMed
  9. Hakim R, et al. 2019. Stem Cell Res Ther. 10:115. PubMed
  10. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  11. Wu J, et al. 2019. J Immunol Res. 2019:1749803. PubMed
  12. Kimura A, et al. 2018. J Exp Med. 215:2197. PubMed
  13. Danne C et al. 2017. Cell host & microbe. 22(6):733-745 . PubMed
  14. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  15. Wang H et al. 2017. Developmental cell. 40(6):566-582 . PubMed
  16. Mulder R, et al. 2017. Front Immunol. 1.464583333. PubMed
  17. Kramer K, et al. 2017. ACS Omega. 0.240972222. PubMed
  18. Orillion A, et al. 2018. Clin Cancer Res. 24:6383. PubMed
  19. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  20. Sivapatham S, et al. 2019. Front Immunol. 1.865277778. PubMed
  21. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  22. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  23. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
  24. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  25. Park CJ, et al. 2020. Cell Reports. 31(2):107496.. PubMed
  26. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  27. Tsukasaki M, et al. 2020. Cell Reports. 32(10):108124. PubMed
  28. Hays C, et al. 2020. eLife. 8:e48772.. PubMed
  29. Steinmann S, et al. 2020. Sci Rep. 1.160416667. PubMed
  30. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  31. Bertino P, et al. 2019. Oncoimmunology. 8:1601482. PubMed
  32. Morris E, et al. 2005. J Clin Invest. 115:3093. PubMed
  33. Kaplan D, et al. 2007. J Exp Med. 204:2545. PubMed
  34. Banovic T, et al. 2009. J Immunol. 182:912. PubMed
  35. Xiao S, Manley N 2010. PLoS One. 5:e15396. PubMed
  36. Dutta S, et al. 2012. Eukaryot Cell. 0.756944444. PubMed
  37. Dong S, et al. 2007. Int Immunopharmacol. 7:725. PubMed
  38. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  39. Steinbach K, et al. 2013. J Immunol. 191:4531. PubMed
  40. Koma T, et al. 2014. J Virol. 88:7178. PubMed
  41. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  42. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  43. White C, et al. 2015. J Immunol. 194:697. PubMed
  44. Stack G, et al. 2015. PLoS Pathog. 11:1004641. PubMed
  45. Harberts E, et al. 2015. J Immunol. 194:3127. PubMed
  46. Koyama M, et al. 2015. J Exp Med. 212: 1303 - 1321. PubMed
  47. Schulz A, et al. 2015. J Biol Chem. 211: 553 - 567. PubMed
  48. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  49. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  50. Miki Y, et al. 2016. J Biol Chem. 291: 15588 - 15601. PubMed
  51. Maisel D, et al. 2016. PLoS One. 11: 0159716. PubMed
  52. Hidaka T, et al. 2016. Nat Immunol. 18:64-73. PubMed
  53. Aguilera T, et al. 2016. Nat Commun. 7:13898. PubMed
  54. Kramer K, et al. 2017. Mol Ther. 25(1):62-70. PubMed
  55. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  56. Aguilera TA, et al. 2020. Clin Cancer Res. 26:2972. PubMed
  57. Wang Y, et al. 2021. Sci Rep. 1.429861111. PubMed
  58. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  59. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  60. Du J, et al. 2020. Developmental Cell. 55(6):737-753.e7. PubMed
  61. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  62. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  63. Kim SH, et al. 2021. Cell Reports. 35(2):108995. PubMed
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RRID
AB_313320 (BioLegend Cat. No. 107605)
AB_313321 (BioLegend Cat. No. 107606)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 10-30-2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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