TruStain FcX™ (anti-mouse CD16/32) Antibody

Pricing & Availability
Clone
93 (See other available formats)
Other Names
Fcγ R III/II, Ly-17
Isotype
Rat IgG2a, λ
Ave. Rating
5 reviews
Product Citations
publications
Cat # Size Price Quantity Avail. Save
101319 50 µg 36€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101320 500 µg 156€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD16 is the low affinity IgG Fc receptor III (FcR III) and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. TruStain FcX™ is specific to the common epitope of CD16/CD32. It is useful for blocking non-specific binding of immunoglobulin to the Fc receptors.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The CD16/32 antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

For blocking of Fc receptors in flow cytometric analysis, pre-incubate the cells with TruStain FcX™ at 1.0 µg per 106 cells in 100 µl volume for 5-10 minutes on ice prior to immunostaining. It is not necessary to wash cells between these blocking and immunostaining steps.

Application References
  1. Oliver AM, et al. 1999. Hybridoma 18:113.
  2. Brummel R and Lenert P. 2005. J. Immunol. 174:2429. PubMed
  3. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349.
  4. Clements JL, et al. 2006. J. Immunol. 177:905. PubMed
  5. Flores M, et al. 2008. FASEB J. 22:3661. PubMed
  6. Ge XN, et al. 2010. J. Immunol. 185:1205. PubMed
  7. Maseda D, et al. 2012. J. Immunol. 188:1036. PubMed
  8. Lewis ND, et al. 2013. J. Immunol. 190:3533. PubMed
  9. Bonne-Annee S, et al. 2013. Infect Immun. 81:3346. PubMed
  10. Parlane NA, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  11. Jarajapu YP, et al. 2014. PLoS One. 9:93965. PubMed
  12. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
  13. Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
  14. Gillis TP, et al. 2014. Infect Immun. 82:3900. PubMed
  15. Minkah N, et al. 2015. J Virol. 89:3366. PubMed
Product Citations
  1. Ge X, et al. 2010. J Immunol. 185:1205. PubMed
  2. Kenyon K, et al. 2011. J Immunol. 187:2101. PubMed
  3. Gerald N, et al. 2011. PLoS One. 6:e24398. PubMed
  4. Maseda D, et al. 2012. J Immunol. 188:1036. PubMed
  5. Lewis N, et al. 2013. J Immunol. 190:3533. PubMed
  6. Bonne-Année S, et al. 2013. Infect Immun . 61:3346. PubMed
  7. Parlane N, et al. 2013. Vet Immunol Immunopathol. 30:122. PubMed
  8. Jarajapu Y, et al. 2014. PLoS One. 9:93965. PubMed
  9. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  10. Resa-Infante P, et al. 2014. J Virol. 88:8166. PubMed
  11. Gillis T, et al. 2014. Infect Immun . 82:3900. PubMed
  12. Minkah N, et al. 2015. J Virol. 89:3366. PubMed
  13. Cieniewicz B, et al. 2015. J Virol. 89: 6562 - 6574. PubMed
  14. Ermert D, et al. 2016. PLoS Pathog. 11: 1005043. PubMed
  15. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  16. Sido J, et al. 2015. J Leukoc Biol. 98: 435-447. PubMed
  17. Lammers K, et al. 2015. PLoS One. 10: 0138338. PubMed
  18. Wang L, et al. 2015. Cancer Immunol Res. 3: 1030-1041. PubMed
  19. Kunisawa J, et al. 2015. Immunity. 1: 122-131. PubMed
  20. Ali A, et al. 2015. J Infect Dis. 212: 1308 - 1316. PubMed
  21. Frost E, et al. 2015. J Immunol. 195: 3520 - 3524. PubMed
  22. Huang W, et al. 2015. J Allergy Clin Immunol. . PubMed
  23. Stutchfield B, et al. 2015. Gastroenterology. 149: 1896-1909.e14. PubMed
  24. Yang Y, et al. 2016. Nucleic Acids Res. 44: 4174 - 4188. PubMed
  25. Tsaousi A, et al. 2016. PLoS One. 11: 0148873. PubMed
  26. Li Z, et al. 2016. Sci Rep. 6:22143. PubMed
  27. Ferretti M, et al. 2016. Brain Behav Immun. 54: 211-225. PubMed
  28. Samuelson D, et al. 2016. J Immunol. 196: 2655 - 2665. PubMed
  29. Jayaraman P, et al. 2016. PLoS One. 12: 1005490. PubMed
  30. Lykken J, et al. 2016. Blood. 127: 1886-1895. PubMed
  31. Li Z, et al. 2016. Nat Commun. 7: 11394. PubMed
  32. Nakagawa A, et al. 2016. Sci Rep. 6: 25009. PubMed
  33. Gupta P, et al. 2016. PLoS One. 11: 0154725. PubMed
  34. Provine N, et al. 2016. J Virol. 90: 4278 - 4288. PubMed
  35. Samusik N, et al. 2016. Nat Methods. 13: 493-496. PubMed
  36. Vasam G, Joshi S, Jarajapu Y 2016. Sci Rep. 6: 26131. PubMed
  37. Asson-Batres M, et al. 2016. Am J Physiol Heart Circ Physiol. 310: 1773 - 1789. PubMed
  38. Buonomo E, et al. 2016. Cell Rep. 16: 432-443. PubMed
  39. Johnson A, et al. 2016. Mol Metab. 5:506-526. PubMed
  40. Rodriguez-Garcia M, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.72. PubMed
  41. Li D, et al. 2016. Sci Rep. 6:31002. PubMed
  42. Reddy S, et al. 2016. MBio. 7: e00723-16. PubMed
  43. Hey Y, O’Neill H 2016. PLoS One. 11: 0162358. PubMed
  44. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  45. Belmonte L, et al. 2016. Sci Rep. 6:35813. PubMed
  46. Tam H, et al. 2016. Proc Natl Acad Sci U S A. 113: E6639 - E6648. PubMed
  47. Yu S, et al. 2016. Mol Ther. 10.1038/mt.2016.175. PubMed
  48. Galand C, et al. 2016. J Allergy Clin Immunol. 138:1356-1366. PubMed
  49. Reyes J, et al. 2016. Infect Immun . 84(12):3471-3483. PubMed
  50. Ju X, et al. 2016. J Immunol. 197(12):4613-4625. PubMed
  51. Bracamonte-Baran W, et al. 2017. Proc Natl Acad Sci U S A. 114(5):1099-1104. PubMed
  52. Matisz C, et al. 2017. Sci Rep. 7:40631. PubMed
  53. Cederquist C, et al. 2017. Mol Metab. 6(1):125-137. PubMed
  54. Lynch T, et al. 2017. J Mol Cell Cardiol. 102:83-93. PubMed
  55. Chiang N, et al. 2017. J Immunol. 198(2):842-851. PubMed
  56. Cortez-Toledo O, et al. 2017. PLoS One. 12(2):e0171268. PubMed
  57. Cuccarese M, et al. 2017. Nat Commun. 8:14293. PubMed
  58. Beug S, et al. 2017. Nat Commun. 10.1038/ncomms14278. PubMed
  59. Huo M, et al. 2017. FASEB J. 10.1096/fj.201601030R. PubMed
  60. Ramaswamy G, et al. 2017. Sci Rep. 10.1038/srep45140. PubMed
  61. Watanabe K, et al. 2017. PLoS Pathog.. 10.1371/journal.ppat.1006513. PubMed
  62. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  63. Zhao J, et al. 2017. Biochem Biophys Res Commun. 10.1016/j.bbrc.2017.06.149. PubMed
  64. Samuelson D, et al. 2017. PLoS Pathogens. 13(6):e1006426. PubMed
  65. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  66. Eslani M,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5507-5517. PubMed
  67. Chen W,et al. 2017. Sci Rep.. 10.1038/s41598-017-13570-2. PubMed
  68. Krishnasamy K,et al. 2017. Nat Commun. . 10.1038/s41467-017-00953-2. PubMed
  69. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  70. Wartewig T, et al. 2017. Nature. . 10.1038/nature24649. PubMed
  71. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  72. Chen D, et al. 2018. Nat Commun. 9:873. PubMed
  73. Abraham V, et al. 2018. Int J Oncol. 53:488. PubMed
  74. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  75. Bellelli R 2018. Molecular cell. 70:707. PubMed
  76. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  77. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  78. Zöller T, et al. 2018. Int J Mol Sci. 19:E706. PubMed
  79. Thurlow LR 2018. Cell host & microbe. 24:261. PubMed
  80. Edgar LJ 2018. Cell chemical biology. 26(1):131. PubMed
  81. Guo XJ 2018. Immunity. 49:531. PubMed
  82. Sade-Feldman M 2018. Cell. 175:998. PubMed
  83. Sheng W 2018. Cell. 174:549. PubMed
  84. Lu Y 2018. Cancer cell. 33:1048. PubMed
  85. Sokol CL 2018. Immunity. 49:449. PubMed
  86. Jacobson A 2018. Cell host & microbe. 24:296. PubMed
  87. Gentek R 2018. Immunity. 48:1160. PubMed
  88. Miura Y 2018. eLife. 7: e36572. PubMed
  89. Divakaruni AS 2018. Cell metabolism. 28:490. PubMed
  90. Moor AE 2018. Cell. 175:1156. PubMed
  91. Rodda LB 2018. Immunity. 48:1014. PubMed
  92. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  93. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  94. van Vloten JP, et al. 2019. Mol Ther Methods Clin Dev. 13:154. PubMed
  95. Contijoch EJ, et al. 2019. Elife. 8:. PubMed
  96. Tavazoie MF, et al. 2018. Cell. 172:825. PubMed
  97. Funk KE, et al. 2019. J Neuroinflammation. 16:22. PubMed
  98. Yrlid U, et al. 2019. J Leukoc Biol. 105:195. PubMed
  99. Wong EL, et al. 2018. Brain Behav Immun. 67:257. PubMed
  100. Schappe MS, et al. 2018. Immunity. 48:59. PubMed
  101. Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed
  102. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  103. Koizumi SI, et al. 2018. Nat Commun. 9:5344. PubMed
  104. Freemerman AJ, et al. 2019. J Immunol. 202:1265. PubMed
  105. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  106. Manglani M, et al. 2018. Curr Protoc Immunol. 121:e44. PubMed
  107. Dillon MT, et al. 2019. Clin Cancer Res. 25:3392. PubMed
  108. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
  109. Agelidis A, et al. 2019. Front Immunol. 10:500. PubMed
  110. Misumi I, et al. 2019. Cell Rep. 27:514. PubMed
  111. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  112. Mitchell LA, et al. 2019. Oncotarget. 10:2252. PubMed
  113. Misumi I, et al. 2019. Cell Rep. 27:1387. PubMed
  114. Tsuchiya K, et al. 2019. Nat Commun. 10:2091. PubMed
  115. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
  116. Bommareddy PK, et al. 2019. J Biol Methods. 6:2. PubMed
  117. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  118. Maximov V, et al. 2019. Nat Commun. 10:2410. PubMed
  119. Eslani M, et al. 2018. Stem Cells. 36:775. PubMed
  120. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  121. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  122. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  123. Axelrod HD, et al. 2019. Mol Cancer Res. 17:356. PubMed
  124. Li B, et al. 2018. Cancer Immunol Res. 6:539. PubMed
  125. Tanigawa Y, et al. 2019. Nat Commun. 10:4064. PubMed
  126. Haupt F, et al. 2019. Sci Rep. 9:9798. PubMed
  127. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  128. Gomez D, et al. 2018. Nat Med. 24:1418. PubMed
  129. Sweere JM, et al. 2019. Science. 363. PubMed
  130. Wirsching HG, et al. 2019. JCI Insight. 4. PubMed
Publication Library
RRID
AB_1574973 (BioLegend Cat. No. 101319)
AB_1574975 (BioLegend Cat. No. 101320)

Antigen Details

Structure
Ig superfamily, 40-60 kD
Distribution

B cells, monocyte/macrophages, NK cells, neutrophils, mast cells, dendritic cells

Function
Low affinity receptors for IgG
Ligand/Receptor
IgG
Cell Type
B cells, Dendritic cells, Macrophages, Mast cells, Monocytes, Neutrophils, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules, Fc Receptors
Gene ID
14130 View all products for this Gene ID 14131 View all products for this Gene ID
UniProt
View information about CD16/32 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11-30-2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account