PE anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class II
Isotype
Rat IgG2b, κ
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Product Citations
publications
M5/114.15.2
C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
  • M5/114.15.2
    C57BL/6 mouse splenocytes were stained with anti-mouse I-A/I-E (clone M5/114.15.2) PE (solid line) or rat IgG2b, κ PE isotype control (dashed line).
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Cat # Size Price Quantity Avail. Save
107607 50 µg 60€
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107608 200 µg 216€
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Application References
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Espinosa-Cueto P, et al. 2017. PLoS One.. 10.1371/journal.pone.0182126. PubMed
  2. Platteel A, et al. 2017. Vaccine.. 10.1016/j.vaccine.2017.10.044. PubMed
  3. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  4. Dhandapani R, et al. 2018. Nat Commun. 9:1640. PubMed
  5. Biton M et al. 2018. Cell. 175(5):1307-1320 . PubMed
  6. Jenkins RW, et al. 2018. Cancer Discov. 8:196. PubMed
  7. Lin SY, et al. 2017. Autophagy. 14:778. PubMed
  8. Shi L, et al. 2019. Theranostics. 9:4115. PubMed
  9. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  10. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  11. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  12. Ioanna E Galani et al. 2017. Immunity. 46(5):875-890 . PubMed
  13. Cai W, et al. 2020. J Immunol Res. 2019:2835256. PubMed
  14. Renner K, et al. 2020. Cell Reports. 29(1):135-150.e9.. PubMed
  15. Kisielow J, et al. 2019. Nat Immunol. 1.286111111. PubMed
  16. Süß P, et al. 2020. Cell Reports. 30(12):4082-4095. PubMed
  17. Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
  18. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  19. Blumenthal D, et al. 2020. eLife. 9:e55995.. PubMed
  20. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  21. Yasmin N, et al. 2013. J Exp Med. 210:2597. PubMed
  22. Xia S, et al. 2014. J Leukoc Biol. 95:733. PubMed
  23. Markey K, et al. 2014. J Immunol. 192:5426. PubMed
  24. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  25. Pazmandi K, et al. 2014. Free Radic Biol Med. 77:281. PubMed
  26. Fontana C, et al. 2016. J Biol Chem. 291: 7727-7741. PubMed
  27. Zhu J, et al. 2016. Sci Rep. 6:27136. PubMed
  28. Novkovic M, et al. 2016. PLoS Biol. 14: 1002515. PubMed
  29. Cao H, et al. 2020. Front Pharmacol. 11:578943. PubMed
  30. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  31. Perry JL, et al. 2020. ACS Nano. 5.583333333. PubMed
  32. Yi M, et al. 2021. J Hematol Oncol. 14:27. PubMed
  33. Huang L, et al. 2021. Frontiers in Cellular and Infection Microbiology. 10:606340. PubMed
  34. Dong R, et al. 2021. Frontiers in Immunology. 12:620238. PubMed
  35. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  36. Guendel F, et al. 2020. Immunity. 53(5):1015-1032.e8. PubMed
  37. Daemen S, et al. 2021. Cell Reports. 34(2):108626. PubMed
  38. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  39. Han C, et al. 2021. Cell Reports. 34(6):108706. PubMed
  40. Bambouskova M, et al. 2021. Cell Reports. 34(10):108756. PubMed
  41. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
RRID
AB_313322 (BioLegend Cat. No. 107607)
AB_313323 (BioLegend Cat. No. 107608)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 10-30-2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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