Brilliant Violet 605™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1.4_BV605_Ly-6C_Antibody_FC_072313
C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 605™ (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
  • HK1.4_BV605_Ly-6C_Antibody_FC_072313
    C57BL/6 mouse bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 605™ (filled histogram). Open histogram represents non-stained cells. Data shown was gated on the myeloid population.
Compare all formats See Brilliant Violet 605™ spectral data
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128035 125 µL 168€
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128036 500 µL 344€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  2. Sierro F, et al. 2017. Immunity. 47:374. PubMed
  3. Alkhani A, et al. 2020. Sci Rep. 10:7165. PubMed
  4. Miranda K, et al. 2022. iScience. 25:104994. PubMed
  5. Wilden A, et al. 2022. Front Immunol. 13:991295. PubMed
  6. Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
  7. Miller CM, et al. 2020. J Virol. 94:00:00. PubMed
  8. Gawish R, et al. 2022. Elife. 11:. PubMed
  9. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  10. Postat J et al. 2018. Immunity. 49(4):654-665 . PubMed
  11. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  12. Leech JM, et al. 2020. Cell Host & Microbe. 26(6):795-809.e5.. PubMed
  13. Yousif AS, et al. 2020. Immunity. 54(2):235-246.e5. PubMed
  14. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  15. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  16. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  17. Hering L, et al. 2021. Int J Mol Sci. 22:. PubMed
  18. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
  19. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  20. Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
  21. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  22. Kedage V, et al. 2022. MAbs. 14:2040083. PubMed
  23. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  24. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  25. Salei N, et al. 2020. J Am Soc Nephrol. 31:257. PubMed
  26. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  27. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  28. Wessel AW, et al. 2020. Nat Commun. 4.123611111. PubMed
  29. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  30. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  31. Bradley KC, et al. 2019. Cell Rep. 28:245. PubMed
  32. Hassan AO, et al. 2021. Cell Reports. 36(4):109452. PubMed
  33. Tondini E, et al. 2022. NPJ Vaccines. 7:64. PubMed
  34. Garland KM, et al. 2021. Front Immunol. 12:753472. PubMed
  35. Da Silva CG, et al. 2019. Theranostics. 4.878472222. PubMed
  36. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  37. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  38. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  39. Su W et al. 2019. Cancer Cell. 36(2):139-155 . PubMed
  40. Blagih J, et al. 2020. Cell Rep. 30:481. PubMed
  41. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  42. Giampazolias E, et al. 2021. Cell. . PubMed
  43. Alam Z, et al. 2020. Cell Rep. 107825:31. PubMed
  44. Robles-Oteiza C, et al. 2021. Dis Model Mech. 14:. PubMed
  45. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  46. Winkler ES, et al. 2020. Nat Immunol. 21:1327. PubMed
  47. Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
  48. Lai SM et al. 2018. Cell reports. 25(11):3099-3109 . PubMed
  49. Soncin I, et al. 2018. Nat Commun. 9:582. PubMed
  50. Kimball AS, et al. 2020. Thromb Haemost. 120:289. PubMed
  51. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  52. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  53. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  54. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  55. Katzmarski N, et al. 2021. Nat Immunol. 22:1382. PubMed
  56. Mathur AN, et al. 2019. Immunity. 50:655. PubMed
  57. Sharma R, et al. 2021. J Neuroinflammation. 72:18. PubMed
  58. Sheng J, et al. 2021. eLife. 10:00. PubMed
  59. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  60. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
  61. Perez-Cruz M, et al. 2021. PLoS One. e0236216:16. PubMed
  62. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  63. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  64. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  65. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  66. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  67. Di Liberto G et al. 2018. Cell. 175(2):458-471 . PubMed
  68. Young K, et al. 2021. Cell Stem Cell. . PubMed
RRID
AB_2562352 (BioLegend Cat. No. 128035)
AB_2562353 (BioLegend Cat. No. 128036)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12-09-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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