Brilliant Violet 570™ anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1.4_BV570_072611
C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
  • HK1.4_BV570_072611
    C57BL/6 bone marrow cells were stained with Ly-6C (clone HK1.4) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Compare all formats See Brilliant Violet 570™ spectral data
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128029 125 µL 164€
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128030 50 µg 225€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 570™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 570™ excites at 405 nm and emits at 570 nm. The bandpass filter 585/42 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 570™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Sepe JJ, et al. 2022. JACC Basic Transl Sci. 7:915. PubMed
  3. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  4. Li J, et al. 2020. Cancer Discov. . PubMed
  5. Wu X, et al. 2021. Elife. 10:. PubMed
  6. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  7. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  8. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  9. Ajina R, et al. 2021. Cancer Immunol Res. 9:386. PubMed
  10. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  11. Li J, et al. 2018. Immunity. 49:178. PubMed
  12. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  13. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  14. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  15. Sanmarco LM, et al. 2021. Nature. 590:473. PubMed
  16. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  17. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  18. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  19. Xu Y, et al. 2014. Proc Natl Acad Sci U S A. 111:6371. PubMed
  20. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
RRID
AB_10896061 (BioLegend Cat. No. 128029)
AB_2562617 (BioLegend Cat. No. 128030)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12-09-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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