PE/Cyanine7 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_PECy7_083109
C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7
  • HK1dot4_PECy7_083109
    C57BL/6 bone marrow cells stained with HK1.4 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
128017 25 µg 84,00€
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128018 100 µg 212,00€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
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  2. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
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  5. Syed I et al. 2018. Cell metabolism. 27(2):419-427 . PubMed
  6. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  7. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  8. Wu W, et al. 2018. J Am Soc Nephrol. 29:168. PubMed
  9. Niknam S, et al. 2018. Clin Cancer Res. 24:5735. PubMed
  10. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  11. Pascual–García M, et al. 2019. Nat Commun. 10:2416. PubMed
  12. Blecher-Gonen R, et al. 2019. Cell Syst. 8:109. PubMed
  13. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  14. Albarrán–Juárez J, et al. 2018. J Exp Med. 215:2655. PubMed
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  17. Yang YS, et al. 2020. Mol Ther Methods Clin Dev. 17:922. PubMed
  18. Zhang J, et al. 2020. Cell Metabolism. 31(6):1136-1153. PubMed
  19. Rasid O, et al. 2020. Cell Reports. 29(12):3933-3945.e3.. PubMed
  20. Weinstock NI, et al. 2020. Neuron. 107(1):65-81. PubMed
  21. Muri J, et al. 2020. eLife. 9:e53627.. PubMed
  22. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  23. Zhou G, et al. 2019. FASEB J. 33:11396. PubMed
  24. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  25. Clement M, et al. 2014. Proc Natl Acad Sci U S A. 111:1101. PubMed
  26. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  27. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  28. Molina M, et al. 2015. PLoS One. 10:127262. PubMed
  29. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
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  31. Syed A, et al. 2015. Infect Immun . 83: 3428-3437. PubMed
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  34. Moyat M, et al. 2015. Infect Immun . 83: 4217 - 4228. PubMed
  35. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  36. Steiger S, et al. 2015. J Immunol. 195: 5495 - 5502. PubMed
  37. Stein K, et al. 2016. J Leukoc Biol. 99: 231 - 239. PubMed
  38. Albarrán-Juárez J, et al. 2016. Atherosclerosis. 251:445-53. PubMed
  39. Diao N, et al. 2016. Sci Rep. 6:34672. PubMed
  40. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
  41. E O’Koren, R Mathew, D Saban 2016. Sci Rep. 6:20636. PubMed
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  47. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
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  53. Tang CC, et al. 2021. eLife. 10:00. PubMed
  54. Ang QY, et al. 2020. Cell. 181(6):1263-1275.e16. PubMed
  55. Koren N, et al. 2021. Cell Host Microbe. 29(2):197-209.e5. PubMed
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RRID
AB_1732093 (BioLegend Cat. No. 128017)
AB_1732082 (BioLegend Cat. No. 128018)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12-09-2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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