FITC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
Ave. Rating
Submit a Review
Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
See FITC spectral data
Cat # Size Price Quantity Avail. Save
128005 50 µg 60€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
128006 500 µg 200€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Al-Sharea A, et al. 2017. Cell Cycle. 10.1016/j.atherosclerosis.2017.08.010. PubMed
  2. Körner A,et al. 2017. Cell Death Differ.. 10.1038/cdd.2017.177. PubMed
  3. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  4. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  5. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  6. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  7. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  8. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  9. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  10. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
  11. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  12. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  13. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  14. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  15. He J, et al. 2019. J Clin Invest. 130. PubMed
  16. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  17. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
  18. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  19. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  20. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  21. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  22. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  23. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  24. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  25. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  26. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  27. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  28. Caballero S, et al. 2017. Invest Ophthalmol Vis Sci. 58:5164. PubMed
  29. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  30. McAlpine CS, et al. 2019. Nature. 566:383. PubMed
  31. Duan Y, et al. 2019. Circ Res. 125:969. PubMed
  32. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  33. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  34. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  35. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  36. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
  37. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
  38. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  39. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
  40. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  41. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  42. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
  43. Lee S, et al. 2011. J Immunol. 186:6972. PubMed
  44. Alvarez-Breckenridge C, et al. 2012. J Virol. 86:4566. PubMed
  45. Buzas K, et al. 2011. Cytokine. 55:429. PubMed
  46. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  47. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  48. Mirakaj V, et al. 2014. J Exp Med. 211:1037. PubMed
  49. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  50. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  51. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  52. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  53. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  54. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  55. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  56. Cao Y, et al. 2015. J Biol Chem. 290: 23050-23063. PubMed
  57. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  58. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  59. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  60. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  61. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  62. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  63. You Y, et al. 2016. Sci Rep. 6:20630. PubMed
  64. Xu Y, et al. 2016. Arthritis Rheum. 67: 225-237. PubMed
  65. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  66. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  67. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  68. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  69. Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed
  70. Rahtes A, et al. 2020. Front Immunol. 1.484722222. PubMed
  71. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  72. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  73. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  74. Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed
  75. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  76. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  77. Hou PP, et al. 2020. Molecular Cell. 78(6):1192-1206.e10. PubMed
  78. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  79. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  80. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  81. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  82. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  83. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  84. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  85. Dong L, et al. 2021. Cancer Cell. . PubMed
  86. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
RRID
AB_1186134 (BioLegend Cat. No. 128005)
AB_1186135 (BioLegend Cat. No. 128006)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12-09-2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account