FITC anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_FITC_011409.jpg
C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
  • HK1dot4_FITC_011409.jpg
    C57BL/6 bone marrow cells stained with HK1.4 FITC (gated on myeloid cell population)
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128005 50 µg 72€
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128006 500 µg 235€
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Combes F, et al. 2018. Neoplasia. 20:848. PubMed
  2. de la Rosa X, et al. 2018. Am J Pathol. 188:950. PubMed
  3. Ying W, et al. 2019. Cell Metab. 29:457. PubMed
  4. Duan Y, et al. 2019. Circ Res. 125:969. PubMed
  5. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  6. Liu Y, et al. 2021. Cell Metabolism. 33(6):1221-1233.e11. PubMed
  7. Zaman R, et al. 2021. Immunity. :. PubMed
  8. Liang J, et al. 2021. Cancer Manag Res. 13:6977. PubMed
  9. Rosina M, et al. 2022. Cell Metab. 34:533. PubMed
  10. Jones NM, et al. 2019. BMC Cancer. 1.127777778. PubMed
  11. John S, et al. 2020. Sci Rep. 10:11377. PubMed
  12. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  13. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  14. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
  15. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  16. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  17. Keeter WC, et al. 2022. Eur Heart J Open. 2:oeac028. PubMed
  18. Fan L, et al. 2021. Cancers (Basel). 13:. PubMed
  19. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  20. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  21. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  22. You Y, et al. 2016. Sci Rep. 6:20630. PubMed
  23. Dong L, et al. 2021. Cancer Cell. . PubMed
  24. Zhang Z, et al. 2020. J Immunol. 204:3400. PubMed
  25. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  26. Shani O, et al. 2020. Cancer Res. 80:5317. PubMed
  27. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  28. McAlpine CS, et al. 2019. Nature. 566:383. PubMed
  29. Buzas K, et al. 2011. Cytokine. 55:429. PubMed
  30. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  31. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  32. Iwata H, et al. 2016. Nat Commun. 7:12849. PubMed
  33. Uroda T, et al. 2020. Nat Protoc. 15:2107. PubMed
  34. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  35. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  36. Dolgova EV, et al. 2022. Int J Mol Sci. 23:. PubMed
  37. Paprckova D, et al. 2022. Front Immunol. 13:1009198. PubMed
  38. Schlegel M, et al. 2018. J Clin Invest. 128:4711. PubMed
  39. Al-Sharea A, et al. 2017. Cell Cycle. 10.1016/j.atherosclerosis.2017.08.010. PubMed
  40. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  41. Moreau GB, et al. 2020. Am J Trop Med Hyg. 103:1215. PubMed
  42. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  43. Mirakaj V, et al. 2014. J Exp Med. 211:1037. PubMed
  44. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  45. Bhattacharya P, et al. 2022. PLoS Negl Trop Dis. 16:e0010224. PubMed
  46. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  47. Shin SH, et al. 2022. Front Cell Neurosci. 16:844480. PubMed
  48. Xiao P, et al. 2019. J Exp Med. 216:337. PubMed
  49. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  50. Eichin D, et al. 2015. PLoS One. 10: 0134721. PubMed
  51. McAlpine CS, et al. 2021. Nature. 595:701. PubMed
  52. Naler LB, et al. 2022. Commun Biol. 5:102. PubMed
  53. Körner A, et al. 2019. Nat Commun. 10:633. PubMed
  54. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  55. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  56. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  57. Körner A, et al. 2019. Proc Natl Acad Sci U S A. 116:20623. PubMed
  58. Kulkarni U, et al. 2019. Mucosal Immunol. 12:545. PubMed
  59. Suresh MV, et al. 2019. JCI Insight. 4:e133103. PubMed
  60. Han D, et al. 2021. J Immunol Res. 6688053:2021. PubMed
  61. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  62. Cao Y, et al. 2015. J Biol Chem. 290: 23050-23063. PubMed
  63. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  64. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  65. He Y, et al. 2021. Front Immunol. 12:641206. PubMed
  66. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  67. Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
  68. Körner A,et al. 2017. Cell Death Differ.. 10.1038/cdd.2017.177. PubMed
  69. He J, et al. 2019. J Clin Invest. 130. PubMed
  70. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  71. Xu Y, et al. 2016. Arthritis Rheum. 67: 225-237. PubMed
  72. Ma H, Wan S, Xia C 2016. J Leukoc Biol. 99: 1121 - 1129. PubMed
  73. Guldner IH, et al. 2020. Cell. 183(5):1234-1248.e25. PubMed
  74. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
  75. Monteran L, et al. 2022. Nat Commun. 13:5797. PubMed
  76. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  77. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  78. Cohen M et al. 2018. Cell. 175(4):1031-1044 . PubMed
  79. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  80. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  81. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  82. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  83. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  84. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  85. Lee S, et al. 2011. J Immunol. 186:6972. PubMed
  86. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  87. Li D, et al. 2022. Emerg Microbes Infect. 11:2248. PubMed
  88. Gomez S, et al. 2022. J Immunother Cancer. 10:. PubMed
  89. Alvarez-Breckenridge C, et al. 2012. J Virol. 86:4566. PubMed
  90. Hightower RM, et al. 2019. Mol Ther. 28:189. PubMed
  91. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  92. Hou PP, et al. 2020. Molecular Cell. 78(6):1192-1206.e10. PubMed
  93. Xu G, et al. 2020. Cell Rep. 32:108170. PubMed
  94. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  95. León-Cabrera S, et al. 2014. Int J Biol Sci. 9 948 . PubMed
  96. Zhao T, et al. 2022. JCI Insight. 7:. PubMed
  97. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  98. Alexander Mildner et al. 2017. Immunity. 46(5):849-862 . PubMed
  99. Chiang N, et al. 2019. J Clin Invest. 129:5294. PubMed
  100. Liu Y, et al. 2015. BioMed Res Int. 2015 563425. PubMed
  101. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  102. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  103. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  104. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  105. Ji L et al. 2019. Immunity. 51(2):272-284 . PubMed
  106. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  107. Hobbs BE, et al. 2021. Pathogens. 10:. PubMed
  108. Roediger B et al. 2018. Cell. 175(2):530-543 . PubMed
  109. Meng Michelle Xu et al. 2017. Immunity. 47(2):363-373 . PubMed
  110. Caballero S, et al. 2017. Invest Ophthalmol Vis Sci. 58:5164. PubMed
  111. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  112. Knuplez E, et al. 2021. British Journal of Pharmacology. 178(5):1234-1248. PubMed
  113. Donlan AN, et al. 2021. JCI Insight. 6: . PubMed
  114. Tyagi A, et al. 2021. Nat Commun. 12:474. PubMed
  115. Tang S, et al. 2022. Virol J. 19:32. PubMed
  116. Ramanan D, et al. 2020. Cell. 181(6):1276-1290. PubMed
  117. Nguyen HX et al. 2017. J Neurosci. 37(38):9269-9287 . PubMed
  118. Rahtes A, et al. 2020. Front Immunol. 1.484722222. PubMed
  119. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  120. Ngkelo A, et al. 2016. J Exp Med. 213: 1353 - 1374. PubMed
  121. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  122. Asahara M, et al. 2022. PLoS One. 17:e0276135. PubMed
  123. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  124. Lei Y, et al. 2021. Sci Adv. 7:. PubMed
  125. Guan D, et al. 2021. Cell Death Dis. 12:431. PubMed
  126. Tummala G, et al. 2021. Invest Ophthalmol Vis Sci. 62:41. PubMed
  127. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  128. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  129. Hilt ZT, et al. 2019. JCI Insight. 4:5. PubMed
  130. Hegde V, et al. 2015. J Immunol. 194:5211. PubMed
  131. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  132. Tran S, et al. 2020. Immunity. 53(3):627-640.e5. PubMed
  133. Jones GS, et al. 2020. mSphere. 5:. PubMed
  134. Akhand SS, et al. 2020. Cancer Immunol Res. 8:1542. PubMed
  135. Reinke S, et al. 2020. Cell Reports. 30(8):2501-2511. PubMed
  136. Ershaid N, et al. 2019. Nat Commun. 10:4375. PubMed
  137. Abe H, et al. 2019. Nat Commun. 10:2824. PubMed
  138. Galeano Nio JL, et al. 2020. eLife. 9:00. PubMed
  139. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  140. Yang F, et al. 2021. Nat Commun. 12:3424. PubMed
RRID
AB_1186134 (BioLegend Cat. No. 128005)
AB_1186135 (BioLegend Cat. No. 128006)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12-09-2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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