Ultra-LEAF Purified anti-human CD16 Antibody anti-CD16

Pricing & Availability

Clone: 3G8 (See other available formats)
Regulatory Status: RUO
Workshop: V NK80
Other Names: FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human peripheral blood lymphocytes stained with Ultra-LEAF™ purified CD16 (clone 3G8), followed by anti-mouse IgG FITC.

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302049

100 µg

93€

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302050

1 mg

463€

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302057

5 mg

595€

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302058

25 mg

1760€

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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human, Cynomolgus, Rhesus

Reported Reactivity

African Green, Baboon, Capuchin Monkey, Chimpanzee, Common Marmoset, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human PMN cells

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
CyTOF® - Verified
Block, IHC-F, IP, PG, Stim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation¹⁸. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections⁶, immunoprecipitation³, stimulation of NK cell proliferation⁴, blocking of phagocytosis⁵, and blocking of immunoglobulin binding to FcγRIII^7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Application References

Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
Hoshino S, et al. 1991. Blood 78:3232. (Stim)
Tamm A, et al. 1996. Immunol. 157:1576. (Block)
Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)

Product Citations

Yamin R, et al. 2021. Nature. 599:465. PubMed
Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
Waight JD, et al. 2018. Cancer Cell. 33:1033. PubMed
Zhu C, et al. 2020. Front Immunol. 1.688194444. PubMed
Gimpel AK, et al. 2022. Front Immunol. 12:761816. PubMed
Beasley AB, et al. 2021. Methods Mol Biol. 2265:213. PubMed
Thulin NK, et al. 2020. Cell Reports. 31(6):107642. PubMed

RRID

AB_2783156 (BioLegend Cat. No. 302049)
AB_2561481 (BioLegend Cat. No. 302050)
AB_2572005 (BioLegend Cat. No. 302057)
AB_2572006 (BioLegend Cat. No. 302058)

Antigen Details

Structure: Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution: NK cells, activated monocytes, macrophages, neutrophils
Function: Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor: Aggregated IgG, IgG-antigen complex
Cell Type: Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.
Gene ID: 2214 View all products for this Gene ID
UniProt: View information about CD16 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?: Yes
Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD16 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD16	3G8	FC
Biotin anti-human CD16	3G8	FC
FITC anti-human CD16	3G8	FC
Brilliant Violet 711™ anti-human CD16	3G8	FC
PE anti-human CD16	3G8	FC
PE/Cyanine5 anti-human CD16	3G8	FC
Purified anti-human CD16	3G8	FC,CyTOF®,Block,IHC-F,IP,Stim
APC/Cyanine7 anti-human CD16	3G8	FC
PE/Cyanine7 anti-human CD16	3G8	FC
Alexa Fluor® 488 anti-human CD16	3G8	FC
Alexa Fluor® 647 anti-human CD16	3G8	FC
Pacific Blue™ anti-human CD16	3G8	FC
Alexa Fluor® 700 anti-human CD16	3G8	FC
PerCP/Cyanine5.5 anti-human CD16	3G8	FC
PerCP anti-human CD16	3G8	FC
Brilliant Violet 421™ anti-human CD16	3G8	FC
Brilliant Violet 570™ anti-human CD16	3G8	FC
Brilliant Violet 605™ anti-human CD16	3G8	FC
Brilliant Violet 650™ anti-human CD16	3G8	FC
Brilliant Violet 785™ anti-human CD16	3G8	FC
Brilliant Violet 510™ anti-human CD16	3G8	FC
Ultra-LEAF™ Purified anti-human CD16	3G8	FC,CyTOF®,Block,IHC-F,IP,Stim
Purified anti-human CD16 (Maxpar® Ready)	3G8	FC,CyTOF®
PE/Dazzle™ 594 anti-human CD16	3G8	FC
APC/Fire™ 750 anti-human CD16	3G8	FC
PE anti-human CD16	3G8	FC
APC anti-human CD16	3G8	FC
Pacific Blue™ anti-human CD16	3G8	FC
PE/Dazzle™ 594 anti-human CD16	3G8	FC
TotalSeq™-A0083 anti-human CD16	3G8	PG
TotalSeq™-B0083 anti-human CD16	3G8	PG
TotalSeq™-C0083 anti-human CD16	3G8	PG
PE/Cyanine7 anti-human CD16	3G8	FC
PE/Fire™ 640 anti-human CD16	3G8	FC
FITC anti-human CD16	3G8	FC
Spark YG™ 581 anti-human CD16	3G8	FC
APC/Fire™ 750 anti-human CD16	3G8	FC
TotalSeq™-D0083 anti-human CD16	3G8	PG
APC/Fire™ 810 anti-human CD16	3G8	FC
GMP APC anti-human CD16	3G8	FC
GMP PE/Dazzle™ 594 anti-human CD16	3G8	FC
GMP PE anti-human CD16	3G8	FC
Spark Red™ 718 anti-human CD16	3G8	FC
GMP Pacific Blue™ anti-human CD16	3G8	FC
GMP FITC anti-human CD16	3G8	FC
Spark Blue™ 515 anti-human CD16	3G8	FC

Concentration & Expiration Lookup

Certificate of Analysis

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Go To Top Version: 5 Revision Date: 08/16/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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