Purified anti-human CD16 Antibody

Pricing & Availability
Clone
3G8 (See other available formats)
Workshop
V NK80
Other Names
FcγRIII
Isotype
Mouse IgG1, κ
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Product Citations
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3G8_Pure_071805
Human peripheral blood lymphocytes stained with purified 3G8 and anti-mouse IgGs FITC
  • 3G8_Pure_071805
    Human peripheral blood lymphocytes stained with purified 3G8 and anti-mouse IgGs FITC
Cat # Size Price Quantity Avail. Save
302001 25 µg 44€
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302002 100 µg 80€
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Capuchin Monkey, Chimpanzee, Cynomolgus, Marmoset, Pigtailed Macaque, Rhesus, Sooty Mangabey, Squirrel Monkey
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Block, IHC-F, IP, Stim - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the Fc?RIIa and Fc?RIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to Fc?RIII7,8. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302014). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 302050) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Gainers M, et al. 2007. J Immunol. 179:8509. PubMed
  2. Mody P, et al. 2007. Blood. 110:2974. PubMed
  3. Smed-Sörensen A, et al. 2008. Blood. 111:5037. PubMed
  4. Boltz A, et al. 2011. J Biol Chem. 286:21896. PubMed
  5. Edri-Brami M, et al. 2012. PLoS One. 7:e35772. PubMed
  6. Wu Z, et al. 2013. J Virol. 87:7717. PubMed
  7. Winkler M, et al. 2017. PLoS One.. 10.1371/journal.pone.0182427. PubMed
  8. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  9. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  10. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  11. Dutertre CA et al. 2019. Immunity. 51(3):573-589 . PubMed
  12. Lavin Y et al. 2017. Cell. 169(4):750-765 . PubMed
  13. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  14. Urlaub D, et al. 2017. J Immunol. 198:1944. PubMed
  15. Dallari S, et al. 2017. Nat Commun. 8:14830. PubMed
  16. Fasbender F, et al. 2017. Front Immunol. 0.88125. PubMed
  17. Miyamoto DT, et al. 2018. Cancer Discov. 0.533333333. PubMed
  18. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  19. Zhong Q, et al. 2018. J Immunol. 200:3913. PubMed
  20. Eldredge LC, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L49. PubMed
  21. Steffen U, et al. 2020. Nat Commun. 0.541666667. PubMed
  22. Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed
  23. Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. PubMed
  24. Olin A, et al. 2018. Cell. 174:1277. PubMed
  25. Li L, et al. 2019. Cell Stem Cell. 24:271. PubMed
  26. Jog NR, et al. 2018. Front Immunol. 9:2198. PubMed
  27. Auladell M, et al. 2019. Clin Transl Immunology. 8:e01090. PubMed
  28. Frobel J, et al. 2018. Clin Epigenetics. 10:67. PubMed
  29. Ardain A, et al. 2019. Nature. 570:528. PubMed
  30. Boutboul D, et al. 2018. J Clin Invest. 128:3071. PubMed
  31. Ravindran A, et al. 2018. Front Immunol. 9:2193. PubMed
RRID
AB_314201 (BioLegend Cat. No. 302001)
AB_314202 (BioLegend Cat. No. 302002)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Go To Top Version: 3    Revision Date: 05/01/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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